New answers tagged

1 vote

What's pooling in the spread plate technique?

Forming a pool (second definition in link); forming a small amount of liquid on a surface. A la "I knocked my cup over and the coffee formed a small pool on my bench" Basically this results ...
bob1's user avatar
  • 11.7k
2 votes

Why do we choose to use agar instead of agarose in various microbial applications?

Agarose is indeed more expensive than agar because it is purified from agar, i.e. it is agar minus the agaropectin. Agaropectin is heterogeneous, and modified with acidic side-groups. The consequence ...
CaroZ's user avatar
  • 372
0 votes

Does inoculum volume affect spread plate outcome?

To add to the answer by @MikeyC . The spread plate technique for calculation of colony forming units has volume plated as a variable: CFU/ml = (number of colonies x dilution factor) / volume of ...
bob1's user avatar
  • 11.7k
2 votes
Accepted

Does inoculum volume affect spread plate outcome?

Theoretically, for CFU determination the volume of the inoculum shouldn't matter as long as you plate a dilution series and find the plate with a countable number of colonies. It also shouldn't matter ...
MikeyC's user avatar
  • 4,714
0 votes

Is there any alternative to peptone and beef or yeast extract?

There is an article about using cheap substitutes for peptone, one of the main ingredients of culture medium as a nitrogen-source. The authors used coconut cake (meal), ground-nut or gingelly cake ...
Vera Maria's user avatar
1 vote

Extremophile hunting and identifying unknown microbes

If "identification" is your goal, I would suggest a MinION sequencer and associated equipment for library preparation. These are capable of generating large informative reads for e.g. ...
Maximilian Press's user avatar

Top 50 recent answers are included