43 votes
Accepted

Are these microscopic star-like structures on a dead leaf some sort of organism? If so, which one?

These are stellate ("star-shaped") [and possibly peltate ("borne on a stalk")] hairs ("trichomes") of the leaf itself. From Harris & Harris's (2001) ""...
theforestecologist's user avatar
19 votes
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Can anyone ID this microorganism?

Appears to be a ciliate protist called Vorticella. Image source: Wikimedia (Author: Frank fox, 2010) Vorticella always stood out to me because of their long, narrow stalk they use for anchoring with ...
theforestecologist's user avatar
9 votes
Accepted

Star-like organism seen in microscope

It may be a stellate trichome from a plant. You can find other ones by taking swabs from the onion and nearby plants. cool images here wiki info about trichomes
bandybabboon's user avatar
  • 10.4k
7 votes

What are these micrographs of?

Having had a quick read of the report, I have a few comments - first off, this seems to be a second-hand report of something that hasn't actually been observed by the authors. Second, the light ...
bob1's user avatar
  • 12.1k
6 votes
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What do the text annotations in an electron micrograph mean?

The 1.40kX is the zoom (1400x), WD is the working distance (distance between the final lens and the object), ...
WYSIWYG's user avatar
  • 35.6k
6 votes
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To which thing does "two-thirds" refer here?

The use here of "object glass" is equivalent to our current usage of "objective lens" on a microscope. In this case Darwin used a 2/3 lens and the "middle eye-piece" from ...
bob1's user avatar
  • 12.1k
6 votes
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Why low noise / low light camera on microscope?

I've used such cameras for fluorescence microscopy and had to deal with very low-light, high-noise conditions, especially when looking at transient signals like calcium imaging or flavoprotein ...
Bryan Krause's user avatar
  • 45.7k
6 votes
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Why are my bacterial smears disappearing?

Bacteriological smears are a one-and-done scenario. Very few of them are intended for repeat use. Basically, all you can expect is that you observe it and then discard the slide. Wiping the oil off ...
bob1's user avatar
  • 12.1k
5 votes

Which organelle is the easiest to see in a cell?

A quick google revealed this slideshow that says the nucleus is the easiest organelle to see. Generally, this is what is taught in schools worldwide. Note that mature red blood cells don't have a ...
James's user avatar
  • 11.3k
5 votes
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What does fluorogenic mean for fluorescent probes?

The usage of "fluorogenic" is typically applied to probes that are not currently fluorescent but can be, typically by some enzyme action or other reaction. For example, see here, here, or here. ...
Bryan Krause's user avatar
  • 45.7k
5 votes
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Cannot see images beyond a certain magnification with a microscope

This is probably the problem: "We did not prepare the leaf; we just put a small piece of it between two slides.". I suppose you mean you put a leaf fragment between two regular microscope slides, ...
guest's user avatar
  • 254
5 votes

How are new staining protocols designed for microscopy?

This answer addresses the question of how stains were developed historically, which may bring into perspective your overly modern assumptions. Most of these older (but stil very usefull!) staining ...
guest's user avatar
  • 254
5 votes
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convert bright field images to phase contrast or dic microscopy images (one of those three to any of those three)

I highly doubt it; those are imaging techniques that result from the way light interacts with an object. Faking it wouldn't be useful in the context of science as conversion won't tell you anything ...
bob1's user avatar
  • 12.1k
4 votes

Woodland outdoor microscopy advice

1: Yes it is possible 2: It's not as easy as lab conditions because of variable conditions, chances of equipment breaking, especially when small children are involved. 3: You can also look at a ...
Malhar Khushu's user avatar
4 votes
Accepted

How to prevent blobs under the microscope?

At least some of the blobs that you have appear to be air. Seating the coverslip carefully will help to prevent air-bubbles from forming under the coverslip. Lay one edge against the fluid and slowly ...
Michael_A's user avatar
  • 1,305
4 votes

What is going on in these cells?

they might be embryoid bodies: https://en.m.wikipedia.org/wiki/Embryoid_body
4D Neuron's user avatar
  • 360
4 votes

How do I identify the different stages of meiosis under microscope?

ID characteristics that can help you recognize diplotene better: diplotene : the only difference between this phase and Diakinesis is that The centrosomes reach the poles. you can see the photos ...
BlueFoxy's user avatar
  • 384
4 votes
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Tissue identification in vascular bundle sections

Here is a single Helianthus (sunflower) vascular bundle, complements "The" Ohio State University. The xylem in a vascular bundle is generally the easiest to discern. The phloem tissues is ...
bpedit's user avatar
  • 1,351
4 votes

What to look for when buying a light microscope?

My advice is always the same: "first microscope? keep it simple, keep it cheap, but nevertheless: buy something usable". This Olympus GB is, IMHO, "something simple/cheap/usable": These can be ...
guest's user avatar
  • 254
4 votes
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Why shouldn't I clean microscope slides with a paper towel?

The main reason to avoid paper towels, tissues, and etc. is that they shed lint (paper fibers) which will be left behind on your slide and eventually interfere with your prep. They look small to the ...
bjarchi's user avatar
  • 115
4 votes
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Is it possible to identify this microscopic particle?

This is an plain old ice crystal. Welcome to the microscopic world! When water evaporates, high-energy molecules escape, leaving low-energy molecules of water. The droplets cool this way, and they ...
S Pr's user avatar
  • 6,222
4 votes
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Is there any reason to use an upright microscope over an inverted microscope?

As already mentioned in the comments, cost is one of the primary reasons when you're looking at simple light microscopes. Inverted scopes have more complex optics, along with the convenience factor of ...
MattDMo's user avatar
  • 15.3k
4 votes
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Risk with cultures taken from stagnating water?

First off, without knowing the specifics, it is very hard to say, but in general, so long as you are careful and don't ingest (i.e. drink) or breathe in aerosols/droplets of the water, it should be OK....
bob1's user avatar
  • 12.1k
3 votes

Do different species of bacteria have distinct absorption bands?

Yes they do, as demonstrated by the fact that bacteria have different colors ! Moreover, some bacteria are photosynthetic and others aren't; the photosynthetic bacteria have a very specific mechanism ...
Oosaka's user avatar
  • 3,245
3 votes

Why do people remove excess solution after they put on a coverslip?

Neither of the current answers fully answer the question. The amount of liquid underneath a coverslip should be just sufficient to hold the coverslip and sample onto the slide. If there is too much ...
March Ho's user avatar
  • 9,452
3 votes

Cannot see images beyond a certain magnification with a microscope

25X is a mighty strong ocular (eyepiece) lens, certainly for home use. This means with the 40X objective you are already at 1000X overall. It would take a seriously thin, well mounted and well lit ...
bpedit's user avatar
  • 1,351
3 votes

Atomic force microscopy: depth penetration?

AFM is a surface technique, it has no depth. Spatial resolution can be far in excess of nm - there are publications wherein bonds -ewven hydrogen bonds- are shown. Here is a popularized article which ...
The V's user avatar
  • 130
3 votes

Super-resolution imaging in vivo

Since the question was asked, the field has seen tremendous growth and even a Nobel prize has been awarded for Super-resolution microscopy. Since other answers have already touched upon how STED, PALM,...
dmishra's user avatar
  • 214
3 votes

Can living cells be imaged with electron microscopy?

While it may not be possible yet to image live cells with EM, it is possible to image live animals. For one example, Ishigaki et al. (2012) imaged live ticks (Haemaphysalis flava) with scanning ...
vkehayas's user avatar
  • 1,198

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