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Most microscope software provides options for line average or line integrate as a function that helps the signal to noise ratio when taking an image. Averaging sums the lines and then divides by the average number. Integration sums the lines without dividing. They're functionally the same thing just with different scales. I assume it involves summing ...


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This is more than likely better asked in physics SE, however since you contextualized it biologically somewhat I will answer it here. You are correct in your concern. The Rayleigh Criterion and the associated calculations(Rayleigh Scattering) are talking about the minimum separation of 2 light sources to resolve a given object. From this we are able to ...


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