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When I prepare my slide to use immersion oil, do I really need to wet-mount the specimen? Can I do it with just the coverslip? Ideally you shouldn't use either with immersion oil. You should match the mounting media index as closely as possible to the immersion oil index, otherwise you'll reduce the resolution and contrast of your image. See: http://www....


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The answer is that you cannot use the Immersion Oil without water-mounting the coverslip. I tried it. The water-mount "glues" the coverslip to the slide, effectively anchoring it in place. Without doing this, when you add the Immersion Oil, the coverslip will slide around because the oil "grabs" the coverslip. When you pan the stage, the coverslip is ...


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