4
votes
Accepted
Can I pellet DNA back from dissolved state?
No, you cannot pellet dissolved DNA with ultracentrifugation.
Yes, you can recover a pellet with additional treatments, similarly to how you got it in the first place; only instead of your input ...
- 6,192
2
votes
What are the possible reasons to get extra unrecognized band in agarose gel electrophoresis?
Looks like genomic DNA contamination to me.
More importantly, does it really matter? I certainly appreciate the curiosity but, if your objective is cloning, I would just keep going and then do some ...
- 17.6k
1
vote
What are the possible reasons to get extra unrecognized band in agarose gel electrophoresis?
Could be several things...just off the top of my head. Without your vector map and expected sizes it will be almost impossible to say for sure.
You'll probably also have to give us more information ...
- 1,301
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