4 votes

Why is it sometimes difficult to resuspend E. coli in P1?

Some things to consider. If you spinning too fast and too long, that is going to pack the pellet more. You can spin longer at a slower speed and you will notice your pellet is not as tightly packed. I ...
user avatar
  • 3,675
4 votes
Accepted

Can I pellet DNA back from dissolved state?

No, you cannot pellet dissolved DNA with ultracentrifugation. Yes, you can recover a pellet with additional treatments, similarly to how you got it in the first place; only instead of your input ...
user avatar
  • 5,690
2 votes

What are the possible reasons to get extra unrecognized band in agarose gel electrophoresis?

Looks like genomic DNA contamination to me. More importantly, does it really matter? I certainly appreciate the curiosity but, if your objective is cloning, I would just keep going and then do some ...
user avatar
  • 17.5k
1 vote

What are the possible reasons to get extra unrecognized band in agarose gel electrophoresis?

Could be several things...just off the top of my head. Without your vector map and expected sizes it will be almost impossible to say for sure. You'll probably also have to give us more information ...
user avatar
  • 1,279

Only top scored, non community-wiki answers of a minimum length are eligible