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A method to purify small amounts of bacterial plasmids from the cells to get purified DNA at the end.

Minipreps (=small preparations of plasmid DNA) are usually done using the alkaline lysis method:

  • Cells are lysed with a basic buffer containing detergents
  • Proteins and genomic DNA of the bacteria are precipitated and then pelleted using centrifugation
  • The plasmid DNA is either precipitated with Ethanol or bound to the silica membrane of a flow-through column
  • On the column the DNA is washed, if precipitated, the pellet is washed to get rid of the salts used in the process
  • The pure DNA is either eluted from the column or dissolved from the pellet