If you are looking for information on Coronaviruses, we have prepared this FAQ with information on what is on topic on this site and links to some reputable external resources.
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No, you cannot pellet dissolved DNA with ultracentrifugation. Yes, you can recover a pellet with additional treatments, similarly to how you got it in the first place; only instead of your input being homogenized cells, or tissue, or extract, or whatever you used to obtain your DNA, it would now just be your aqueous solution of DNA. For instance, you may ...


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I (mostly) like the definitions given on this page. An ORF is a sequence of DNA that starts with start codon “ATG” (not always) and ends with any of the three termination codons (TAA, TAG, TGA). Depending on the starting point, there are six possible ways (three on forward strand and three on complementary strand) of translating any nucleotide sequence ...


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For a PCR preparation, swing bucket or fixed angle does not make any differences. The only thing you want is that everything is soluble, in the same volume, and homogeneously mixed. The main difference between swing bucket and fixed angle is how the pellet will be formed in the tube. Depending on the type of extraction or purification you are doing it would ...


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First develop a background of basic highschool chemistry, basic biochemistry and basic genetics. What is molecular biology: Molecular biology has 2 definitions. A broader definition is molecular basis of life. That is indistinguishable from biochemistry. The second definition is how does genetic information flows through molecular mechanism. The secod ...


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I'm sure HIV is well studied since as you know it has a small genome and is highly relevent to therapeutic research, but virus regulation can be complicated and not representative of what happens in normal cells. This is why model organisms exist. The yeast Saccharomyces cerevisiae has about 6,000 genes and researchers have systematically deleted nearly ...


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Without seeing the sequence of the RNA-binding protein it is impossible to be sure, but the obvious conclusion is: The RNA-binding protein is a peptidyl-prolyl cis-trans isomerase. It has been known since 1989 that peptidyl-prolyl cis-trans isomerase is a multiple-function protein, also exhibiting properties of the cyclophilins, proteins which bind ...


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I'll keep the answer to this as concise as possible. The major mismatch repair (MMR) pathway used by eukaryotes is the MutS$\alpha$-MutL$\alpha$-dependent MMR. See the diagram below for its mechanism: The pathway initiates when MutS$\alpha$ (Msh2–Msh6) binds to a mismatch. This is followed by binding of MutL$\alpha$ (Mlh1 and Pms2). PCNA then activates MutL$...


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