New answers tagged

1

I can't find a reference to a "TARIS model" in the paper you linked, but we can break down the molecular circuitry of this lineage tag tracking system step-by-step. The authors use a type of Tet expression system in order to tightly control the expression of a hyperactive Sleeping Beauty (HSB) transposase. In the absence of doxycycline, the reverse ...


4

No, p53 and other tumor suppressor proteins do not belong to Cyclin dependent kinase(CDK)family. p53 blocks the cell cycle by promoting the synthesis of Cyclin dependent kinase inhibitors (CKI proteins). These CKI proteins bind with the Cyclin and CDK complex and inhibit the cell cycle at G1 checkpoint. (Image Source: https://www.khanacademy.org/science/...


2

Single and double stranded DNA have different electrophoretic mobilities, ssDNA will move faster on an agarose gel, so if you know the length of your DNA and it moves further than you expect relative to a dsDNA ladder it's probably ssDNA. The hyperchromic effect means that ssDNA also absorbs more strongly than dsDNA, so you can heat up your DNA and if the ...


1

Dominance or Recessiveness is the characteristic of allele, not gene. Particular gene contains data for producing specific protein. Protein produced from one allele can effect protein synthesis of other one. Protein formed from recessive allele can show dominance over the dominant allele, and this is known as Dominant negative. The protein produced ...


4

Molecular mechanisms of incomplete dominance (a.k.a. partial- or semi-dominance) vary. As an example, let's look at snapdragons and morning glories, two flowering plants that both exhibit incomplete dominance relative to flower pigmentation. Snapdragons (Antirrhinum majus) have multiple alleles at the nivea locus. The wild-type nivea transcript (niv+) ...


2

Strictly speaking, incomplete dominance is an interaction between two alleles of the same gene not between two genes. The most common cause of this is the dosage effect. For example, the product of CHS-D gene is an enzyme required for the synthesis of purple pigment anthocyanin in morning glory flowers. If a plant has two functional (A) alleles of this ...


1

Separate from the alternative translation start site mechanism that tyersome describes is programmed translational frameshifting. This is independent of alternative splicing or post-translational modifications, and happens when a ribosome switches reading frames while a protein is already being translated. Typically, this phenomenon is associated with ...


4

There are multiple mechanisms that are known to lead to translation of substantially (or entirely) different proteins from a single mRNA. While these mechanisms are more typically seen in viruses, I'm focusing on examples documented within the endogenous transcriptome of eukaryotes. Alternative translation initiation One process that can lead to different ...


Top 50 recent answers are included