New answers tagged molecular-biology
3
votes
Why does plasmid cloning efficiency go down with increased size of insert?
I suspect, but don't have any proof, that it is the physical difficulty of getting both ends ligated to a single strand - think of it like this:
Your 10 kb is essentially a long string, ligate one end ...
7
votes
Accepted
How does tetracycline transactivator localize to the nucleus? Where is the nuclear localization signal?
Proteins with a molecular weight less than ~30 kDa(*) can diffuse freely through the nuclear pore (see for example here (1) for a review on nuclear transport).
Assuming that the sequence you provide ...
4
votes
What does the gene name "lexA" stand for?
Lee Theriot's X-ray sensitive mutant https://thebradentontimes.com/lee-frances-theriot-p24426-154.htm I was a postdoc with Joseph Greenberg working in the same field at the time
3
votes
Accepted
Do complementary DNA sequences/codons produce the same amino acids?
Transcription is a highly regulated process, cells aren't just producing random RNAs from random stretches of DNA.
The transcription machinery needs to bind to DNA to get started, which requires a ...
5
votes
Accepted
Ischemia-induced deploarization in excitable cells
Inward-rectifying means positive current passes more easily in the inward direction, however it won't flow differently than the membrane voltage and reversal potential dictate. See for example https://...
3
votes
Accepted
The quality of binding sites?
Quality is most likely related to how tightly RNAp binds to conserved sequences in the promoter, and that is related to how closely the actual sequences themselves are to RNAp's "most-preferred&...
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