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3

Short answer: Looking at the plasmid map, yes, this should work. Long answer: It depends on the plasmid. To be able to replicate a plasmid in bacteria, you need a origin of replication (ori). To find out if this is present, have a look at the vector map provided by Addgene: It shows the ori on the left side of the map. Additionally an antibiotic resistance ...


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It seems that the basic method is just a water extraction - soak some powder from your garlic in water for a period of time. Ideally you would determine experimentally the optimal time and temperature for this using a quantitative method (e.g. HPLC or a biochemical assay of some sort), but you probably don't have access to those. Here's an example of the ...


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No. I'll just use one example, the retina, for a proof by contradiction. In the retina, the principle "forward"/unidirectional pathway would be from photoceptor cells (rods/cones) to bipolar cells (BC) to retinal ganglion cells (RGCs/GCs). RGCs are the projection neurons whose axons make up the optic nerve from eye to the rest of the brain. However,...


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