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1

Historically speaking, the use of VSVG (Vesicular Stomatitis Virus G protein) labeled with GFP was indeed a practical method to define the secretory pathway. (@tyersome comment) However, I am concerned that by the time everything is set up, the cell will be at steady state, which means GFP signal would be present throughout the secretory pathway, preventing ...


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THis was too long for a comment. It would be helpful for you to indicate what exactly you mean by "cells can still function normally and grow". There are a few observations related to your question about absence of centrioles (from your wiki page): In Drosophila mitosis and development can occur to some extent in centriolar mutants, but "they ...


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Adding a third viewpoint, I'm primarily an electrophysiologist in neuroscience. In my corner of the world, I have to say we can be a bit sloppy about terminology from molecular biology. There might be more precise meanings intended in other areas, but when someone writes "protein expression" I typically translate this roughly to "we measured ...


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Nothing is 100% precise - any measurement or process allow for some error, the only difference is how often such errors occur (i.e., the probability of an error). These wildely range in biology - e.g., it is about 1 per $10^4$ in HIV replication, but only 1 per $10^9$ for human DNA (due to the repair mechanisms). Note that $1$ in $10^9$ for human DNA means ...


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There are no formal definitions accepted in science for ambiguous phrases such “protein expression”. If you wish to know what a particular author who uses such a shorthand phrase means you need to examine the context. If you wish to write scientific reports that are comprehensible to others you must express yourself precisely, defining any shorthand phrases ...


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The Thermo Fisher definition is more explicitly accurate, although one could argue that the term "production" in the NCI definition implies regulation and post-translational modification (PTMs) as well. Either way, protein expression definitely includes regulation. Typically, when you measure protein expression, such as via Western blot, you are ...


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What "topologically continuous" appears to mean in this context is that materials can flow between them. So far as I am aware, this is not a well-defined mathematical term, which is part of what makes this confusing. In topology, some spaces are continuous and others are not, but the meaning of "continuous" in topology is rather more ...


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The PURExpress system from NEB is incredibly expensive, for this reason I have avoided it all costs during my experiments with cell-free systems. This is the paper I've used to create my own cheaper cell-free systems while also discussing reasons why using the NEB PURExpress system is not recommended: https://pubs.acs.org/doi/10.1021/acssynbio.8b00427 "...


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