New answers tagged molecular-biology
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Personally, I find these distinctions a bit silly but I also understand they have some use.
People sometimes divide neuroscience into molecular, cellular, and systems levels of understanding. Molecular covers all the "one cell" phenomenon: expression of particular receptors or related proteins, for example. Cellular is one step above of molecular and ...
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the trick is ovum come with a whole package of signal pathways built in before fertilization that you need to trigger implantation and embryonic development. In theory you could engineer these (search artificial ovum) but it is drastically easier and less expensive to just use existing ovum organisms produce for essentially free.
could you use ovum and ...
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The activators are proteins that bind to specific DNA sequences in the enhancer region. These activators also bind to the RNA polymerase and other associated members of the transcription complex, which makes it more likely for an RNA polymerase to hang around the promoter sequence of the region it enhances.
This in turn increases the chance of the gene ...
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Assuming that by termination signal you mean transcription termination signal (and not a stop codon), you would have one shorter RNA molecule. You can have two shorter RNA molecules if there is another transcription start site after the termination signal, but there is no reason to assume this will be the case.
In some cases the RNA polymerase will skip a ...
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Without the sequence information I can only give very general advice.
1) Pick a different location — primers generally work best when they have something close to 50% GC content.
2) Make sure the 3' ends of your primers aren't complementary.
There is detailed advice on how to design effective primers available online including:
from MIT
Dieffenbach, C. ...
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