New answers tagged

3 votes

Why does plasmid cloning efficiency go down with increased size of insert?

I suspect, but don't have any proof, that it is the physical difficulty of getting both ends ligated to a single strand - think of it like this: Your 10 kb is essentially a long string, ligate one end ...
user avatar
  • 7,188
7 votes
Accepted

How does tetracycline transactivator localize to the nucleus? Where is the nuclear localization signal?

Proteins with a molecular weight less than ~30 kDa(*) can diffuse freely through the nuclear pore (see for example here (1) for a review on nuclear transport). Assuming that the sequence you provide ...
user avatar
  • 628
4 votes

What does the gene name "lexA" stand for?

Lee Theriot's X-ray sensitive mutant https://thebradentontimes.com/lee-frances-theriot-p24426-154.htm I was a postdoc with Joseph Greenberg working in the same field at the time
user avatar
3 votes
Accepted

Do complementary DNA sequences/codons produce the same amino acids?

Transcription is a highly regulated process, cells aren't just producing random RNAs from random stretches of DNA. The transcription machinery needs to bind to DNA to get started, which requires a ...
user avatar
  • 36k
5 votes
Accepted

Ischemia-induced deploarization in excitable cells

Inward-rectifying means positive current passes more easily in the inward direction, however it won't flow differently than the membrane voltage and reversal potential dictate. See for example https://...
user avatar
  • 36k
3 votes
Accepted

The quality of binding sites?

Quality is most likely related to how tightly RNAp binds to conserved sequences in the promoter, and that is related to how closely the actual sequences themselves are to RNAp's "most-preferred&...
user avatar
  • 14.7k

Top 50 recent answers are included