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SUIT-2 cell lines are derived from the liver metastases of Pancreatic Ductal Adenocarcinoma. The subline S2-007 is highly metastatic. The other sublines are S2-013, S2-020 and S2-028 which have decreasing metastatic capabilities. You can probably refer these articles for more info : 1. https://www.ncbi.nlm.nih.gov/pubmed/3102439 2. https://link.springer.com/...


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Maybe you can take a look at this. I found it through google when searching for the manual as well. http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006051.pdf


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The ENCODE consortium classifies human and mouse genome elements to try to answer your second question. Work by ENCODE suggested up to 80% of the genome is functional in some way. In other words, by that upper-bound measurement, there is apparently more to DNA's role in biology than just encoding genes — there's a lot going on under the hood. Case for ...


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First, please note that the article you link is not a peer reviewed article. It is a popular, easy-to-read article. Does this article call into question the theory of evolution? No, it does not. What makes you think it does? The article talks about a very old (and somewhat still ongoing) debate about the relative importance of genetic drift vs selection ...


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There are several enzymes involved in amino acid biosynthesis. These some of thse enzymes are encoded in the human genome. You can check out KEGG for a detailed pathway in different organisms. The green arrows denote reactions (and enzymes) that are present in a given organism (Homo sapiens, in this case). If you click on the arrows you can know the ...


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I think you are almost right. DNA (in the nucleus) is transcribed to mRNA, and protein synthesis occurs on ribosomes where the the sequence of bases in the mRNA is translated into protein. Ribosomes are located in the cytoplasm or attached to the endoplasmic reticulum. (The 'mRNA story' is well told in Who discovered mRNA?) But there is also another type of ...


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Your column manual will probably come with buffer recommendations (these manuals are also often available online). You can also search for publications that have purified IgY by anion exchange chromatography and use their procedure as a starting point. Some generalities to keep in mind for buffer selection: Buffers are most effective when the desired pH is ...


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MD is used for studying structural changes. Simulation of biochemical pathways involves the kinetics (you are not really looking at how enzyme structure changes when it binds to substrate). You can certainly pool all the modelling strategies together to make a hybrid multiscale model. There are many people who work on such kind of modelling approaches. You ...


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If you want mitotic chromosomes then you want to look at apical meristems — probably the primary root from germinating seedlings will be easiest. Apical meristems are at the growing tips of roots (and shoots) and contain regions of rapidly dividing cells. Since you can only see chromosomes as separate bodies when they are condensed for cell division this ...


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The terminology of "Rb is active/inactive" refers to the activity of inhibiting E2F. So active Rb inhibits E2F, while inactive Rb doesn't inhibit E2F. With regard to Rb and phosphorylation - the story seems to be more complex ([1] describes Rb as an enigma). According to [2] (this quote is from the summary of the paper by eLife): Narasimha, Kaulich, ...


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