Stack Exchange Network

Stack Exchange network consists of 175 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

New answers tagged

0

The study {1} was published earlier this month and pointed out some side effects of the CCR5-∆ 32 mutation: Abstract: We use the genotyping and death register information of 409,693 individuals of British ancestry to investigate fitness effects of the CCR5-∆ 32 mutation. We estimate a 21% increase in the all-cause mortality rate in individuals who ...


0

Answer is no. Because each CRIPSR-Cas(xx) system works as a ribonucleoproteic complex, so they needs to be loaded with a crRNA in order to be working. Functionality is guaranteed while each of the Cas endonuclease domain is associated with a crRNA. You maybe could use multiple Cas13 with multiple crRNA. In molecular diagnosis is common to use orthogonal ...


2

Yes, it can be determined by sequencing but the sequencing read has to be long enough to span both the mutations. PacBio sequencing allows long reads but it also has higher error rate than the short read sequencing platforms like Illumina. If you know the gene locus then you can amplify the region using PCR and use Sanger sequencing too (for this the ...


2

There are several differences, both mechanistically and functionally. DNA edits are heritable by the daughter cells and are therefore permanent whereas RNA edits are not. The type of Cas protein used in the editing process for DNA and RNA are different. In the paper that you linked the authors have fused an RNA editing enzyme (ADAR) with dCas13 (a variant ...


4

Epigenetic marks are reversible (you might be aware of induced pluripotent cells). Many animals can regenerate organs with high tissue complexity (such as a limb) and this involves de-differentiation in some species (Sandoval-Guzman et al., 2014). Even otherwise, cells can respond to extracellular/environmental cues to modify their epigenetic state (for e.g. ...


2

At this point in time it appears that no known organisms use amino acids beyond the standard repertoire of the 20+2 common amino acids (the +2 being selanocysteine and pyrrolysine) to construct proteins directly from the ribosome. The extensive review provided by Ann Rev Genetics 2017 mentions only selanocysteine and pyrrolysine, and no other amino acids ...


Top 50 recent answers are included