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I assume you are only interested in the comparison of rA-dT (RNA:DNA) and rA-rU (RNA:RNA) duplexes, and not more exotic options involving deoxyuridine (dU) and ribothymidine (m5U). In my mind, there are three likely reasons why oligo-dT may be preferred over oligo-rU for poly(A) hybridization and mRNA capture: The rA-dT duplex is more stable than the rA-rU ...

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