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33 votes

Do all humans have an identical nucleotide sequence for certain proteins, e.g haemoglobin?

It is highly unlikely that there exist any protein that is made from completely identical nucleotide sequences across the entire human population. There will certainly be regions within a gene that ...
Gen Test's user avatar
  • 431
23 votes
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Do all humans have an identical nucleotide sequence for certain proteins, e.g haemoglobin?

Humans have many variants There is variation. The project I use to help understand this natural variation is gnomAD. Using VarMap and a slightly out of date gnomAD file, I counted 16007805 protein-...
James's user avatar
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8 votes
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According to an online course, ribose and adenine can bond to make ATP. Is this true?

The structure shown is indeed of guanine, not adenine. A nucleotide is defined as being composed of a nitrogenous base, a pentose sugar and phosphate (see, e.g. the Wikipedia entry). So the simplest ...
David's user avatar
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8 votes

Do all humans have an identical nucleotide sequence for certain proteins, e.g haemoglobin?

At the whole-gene level, there is likely no absolute conservation of any human protein-coding gene at the population level, though there might be complete conservation between individuals. Keep in ...
acvill's user avatar
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5 votes
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Numbering of C atoms of pyrimidine rings in nucleosides

Although this question should probably have been asked on SE Chemistry, as the molecules are of biochemical importance — and the question is based on a misconception — I will answer it here. Answer ...
David's user avatar
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5 votes

What are the reasons for using oligo-dT instead of oligo-U to isolate mRNAs?

Note - This answer only covers the comparison of rA-dT (RNA:DNA) and rA-rU (RNA:RNA) duplexes, and not more exotic options involving deoxyuridine (dU) and ribothymidine (m5U). I can think of three ...
acvill's user avatar
  • 8,296
5 votes

Chemistry of phosphodiester bond formation by DNA polymerase

I found this paper, which goes very deep into the molecular details of the individual steps of this reaction and also discusses how this is coupled to nucleotide selectivity. The 'basic' details ...
Nicolai's user avatar
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4 votes
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DNA methylation and the validity of the definition of epigenetics

A methylated nucleotide is the same nucleotide, for the purposes of base-pairing events. The methylated base will be paired with its Watson-Crick opposite after replication, for instance (and ...
Alex Reynolds's user avatar
3 votes
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A question about dna triplex

Possibly, yes, but there are complications. There are other conditions that help create the energetically-favorable conditions for bases to bind in a way to form a triplex, including acidity and ...
Alex Reynolds's user avatar
3 votes
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Regarding different nucleotides on a DNA strand

These are IUPAC codes for so-called "degenerate" or ambiguous bases, alternatives to the usual A, T, ...
Alex Reynolds's user avatar
2 votes
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Mutation rate breakdown by original and mutated nucleotide for Coronaviridae

I finally found a paper with numbers, it is Host-directed editing of the SARS-CoV-2 genome by Tobias Mourier, Mukhtar Sadykov, Michael J.Carr, Gabriel Gonzale, William W. Hall, and Arnab Pain. The ...
Sir Cornflakes's user avatar
1 vote
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How do you find the template strand for a sequence where start codon is in both strands?

In real life, you can't predict the start of a protein by looking for ATG. in organisms, the shape of the DNA (which is based in part on sequence) determines where transcription or translations ...
swbarnes2's user avatar
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1 vote

Could p-loop bind fully protonated ATP?

A really simple answer is that hydrogen bonds are stronger when the hydroxyl groups on the phosphates are deprotonated, because hydrogen bonds are like a competition for a proton, but, hydrogen bonds ...
KanyeWest2's user avatar
1 vote

Does Remdesivir cause bone marrow suppression?

From Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral Agent: Overall, the cellular and biochemical assays demonstrated a low potential for RDV to elicit off-...
timeskull's user avatar
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1 vote

How does the position of hydroxyl group in a nucleotide monomer affect the dehydration synthesis of nucleotides?

Wherever the hydroxyl group is; the result is the same I think. The phosphate has oxygen arranged in a tetrahedron around it. In cells the hydrogen is not attached most of the time. For example the ...
Polypipe Wrangler's user avatar
1 vote

Does DNA have a phosphate group or phosphoric acid?

The phosphate ion has a negative formal charge and in an acidic solution its appears as a conjugate base of a phosphoric acid. That is the wey the phosphate group found in cells. The nucleotide ...
Armand's user avatar
  • 35
1 vote

DNA methylation and the validity of the definition of epigenetics

The Methylation will change the individual nucleotide(s) and persist after replication. However the order of the base pairs does not change, so the information in the DNA will still remain , and the ...
Thomas's user avatar
  • 156
1 vote

What is the difference between nucleotide polymorphism (θ) and nucleotide diversity (π)?

You are also confusing definition of these terms with their expected values (typically computed from coalescent theory). Let's look at an example. Consider a haploid population of 6 individuals. We ...
Remi.b's user avatar
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1 vote

flavivirus virus genome, methylated guanine

Why do you think the methylated G is present in these sequences? I have two reasons for thinking your question isn't answerable. As I said in my comment, based on BLAST results I don't think your ...
tyersome's user avatar
  • 5,598
1 vote

Chemistry of phosphodiester bond formation by DNA polymerase

Apologia The answer given by @Nicolai is essentially correct (and I have upvoted it). However I feel that the question embodies certain mistaken assumptions that should be challenged so that naïve ...
David's user avatar
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