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PCR: Are primers designed to be complementary to a DNA sequence within the template DNA or to the 3' ends of the template DNA?

You seem to be confused about the template DNA having a relevant "end". Most DNA molecules one would start with would be very long, often an entire chromosome. The 3' ends of the two ...
mgkrebbs's user avatar
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What should be considered a GC clamp in a qPCR primer?

I have never heard it being called a "GC clamp" but designing primers with GC near the 3' end is a standard way to make the 3' end duplex more stable, allowing for greater efficiency but ...
mmhryc's user avatar
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