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10 votes
Accepted

Saturated Mutagenesis Screening

This sort of method is indeed quite useful and frequently used in synthetic biology: I've used a similar approach before to generate 5' insulators for promoters. Calculating the exact theoretical ...
jakebeal's user avatar
  • 6,987
6 votes

Sheep vs. Furry animals

Fur, wool, and hair are all made of keratins. To the best of my knowledge wool and fur are separated arbitrarily, based on the properties of the fibres. This arbitrary division allows rabbits to ...
Michael_A's user avatar
  • 1,305
4 votes
Accepted

Could AI be applied to protein folding?

Yes, and no :-) In the meantime many protein structures can be predicted quite accurately - even those for which no reference fold had been known before. In this case the important buzz word is "...
tsttst's user avatar
  • 1,597
3 votes
Accepted

what is the meaning of VIP-cre::LSL-TOM::SOMGFP(GIN) mice?

I think I can help explain all but your 3rd question. A transgenic mouse will typically have the genetic construct in all cells. Unless you get a chimeric animal, which is screened for during the ...
akaDrHouse's user avatar
  • 1,309
3 votes

What is protein folding and how is it relevant to disease?

Protein folding is a complex thing. There are huge computer algorithms and huge mainframes which are trying to predict the final 3D structure of a protein. Knowing the tertiary and the quaternary ...
flavinsky's user avatar
  • 186
2 votes

Why are camelid-derived nanobodies called VHH (variable heavy domain of heavy chain)?

First, the definition of VHH (and VH) from the article by S Muyldermans — “Nanobodies: Natural Single-Domain Antibodies” in Annu. Rev. Biochem. 2013. 82:775–97. VHH: antigen-binding variable domain ...
David's user avatar
  • 25.8k
2 votes
Accepted

Are there any small peptides with protease activity?

60 residues is the smallest I could find, ref below. https://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-19-54
Polypipe Wrangler's user avatar
1 vote
Accepted

Is it feasible to grow edible crops for lawns?

There are lots of things that you can eat that you could grow as a lawn. That probably are growing in your lawn now. My yard is about half violets and they are edible. Purslane is a delicious ...
Willk's user avatar
  • 2,964
1 vote

Computational engineering of protein binding pocket

You can try an evolutionary algorithm. If you can quickly evaluate the binding affinity you can initialize a set of copies of your protein but with random mutations. You can assign them a fitness ...
Carlos G. Oliver's user avatar
1 vote

What is protein folding and how is it relevant to disease?

An article, Protein Misfolding and Degenerative Diseases, covers this question, and although it focuses on a single type of disease, this is of great importance. To summarize, there is the potential ...
David's user avatar
  • 25.8k

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