3 votes
Accepted

Selection of recombinant host by color selection method

The Z gene you are talking about should be lacZ that encodes the $\beta-$galactosidase, which is made from $\alpha$ and $\omega$ peptides. Neither peptide is functional by itself. $\beta-$...
Macrophage's user avatar
3 votes

Vectors for genetic engineering

Yes, Agrobacterium is indeed a very widely used vector in plants. So it wouldn't be wrong to consider bacteria as vectors. Just to add, it's worth noting that in recent times, bacterial vector options ...
physics12's user avatar
1 vote

Help with gene linkage and mapping!

I think your reasoning is sound and your ordering very likely, but I wouldn't feel comfortable with deciding the side B is on. Actually, it's more than that: I think that by using basically the same ...
allepasse's user avatar
  • 341
1 vote

use of antibiotics in selection of good plasmids

Short answer: selection protocols are designed to work When choosing a selectable marker for a plasmid, one chooses a gene that is known to be absent in the host organism of interest. For example, ...
J--'s user avatar
  • 428
1 vote

Are restriction sites added on to the ends of a DNA fragment or are they just conveniently there?

That depends a bit on the method you are using, but mainly you are right, it is rather unlikely that a piece of DNA contains the right restriction site on both ends, let alone even two different ones ...
Chris's user avatar
  • 51.6k
1 vote

Recombination Data Set

b+/c+ b/c b+/c b/c+ Ada ~0.39 ~0.42 ~0.08 ~0.1 Donald ~0.09 ~0.09 ~0.4 ~0.41 The other chromosome beeing b/c (I assume each one got an heterozygote fly and than they did a testcross) There are a ...
Hachiloni's user avatar
  • 973
1 vote

Gene of interest number of copies in transformed K. pastoris

To predict the number integrations is probably not possible, but you can favor multiple integrations, if that's what you are after. At least in Saccharomyces cerevisiae integration of a first plasmid ...
gaspanic's user avatar
  • 628
1 vote

Vectors for genetic engineering

Agrobacterium tumefaciens and Agrobacterium rhizogenes are soil-based plant pathogenic bacterial strains containing plasmid. This plasmid is known as Ti plasmid and is responsible for inducing tumor. ...
Twinkle Sheen's user avatar
1 vote

Why are two forward and reverse priming sites depicted here? what do they do?

It's just extra information that you don't need to understand the part above. You could use these primers for colony PCR or sequencing when you'd use the plasmid (after transforming to E.coli for ...
VonBeche's user avatar
  • 1,473
1 vote

Pichia pastoris and ornithine

Yes, it contains arginase. Thise enzyme converts arginine to ornithine and is probably part of the urea cycle. You might notice the arginase is listed as coming from Komagataella phaffii GS115, but ...
VonBeche's user avatar
  • 1,473
1 vote
Accepted

Relative concentration of enzyme vs reaction product

Remember that the concentrations you report are in mass units rather than mole units. If the protein has a mass of 40,000 Da, reasonable for many proteins, then 20 mg/liter means $0.5$ x $10^{-6}$ mol/...
EdM's user avatar
  • 710

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