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Why using Fokl as an enzyme in the zinc finger nuclease method?

In short, yes, there are restriction enzymes that induce double stranded breaks. In fact, unmodified FokI is a restriction endonuclease (indicating it creates double-stranded breaks) ¹. Modified ...
Olaf's user avatar
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4 votes
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Change of DNA concentration due to restriction digest?

Summary The poster wishes to know why spectrophotometric readings (presumably absorbance at 260 nm) on a sample of genomic DNA from an unspecified organism appear “slightly” greater after incubation ...
David's user avatar
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3 votes

Change of DNA concentration due to restriction digest?

As you did a clean up, then you lost some DNA in this process. There are always losses from clean up steps. Note also that spectrophotometry is notoriously unreliable for DNA quantitation; it's more ...
bob1's user avatar
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