9 votes
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Do any RNAs directly inhibit transcription

Yes there are reports of RNA directly inhibiting transcription. RNA induced transcriptional silencing (RITS) is a well known pathway in Schizosaccharomyces pombe (fission yeast). Initial ...
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6 votes

Are non-coding RNAs introns?

A clarification on introns and exons. While it is true that introns are not a part of the mRNA as March Ho said, they are essentially transcribed. This may seem trivial but it is important to note. ...
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6 votes

Are non-coding RNAs introns?

While Ankur's answer is correct, it must be noted that not all non-coding RNAs are introns. An intron must be excised from an mRNA, which therefore means that any non-coding RNA that is not part of ...
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5 votes
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How much time do the different mechanisms of gene regulation need to take effect?

People often imprecisely say that type of regulation takes place on the order of many minutes to hours, and that may be as precise as you can get given the variable kinetics of any given pathway. ...
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  • 470
3 votes
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Which mRNAs enter P-bodies?

I don't really understand why mRNA processing doesn't simply occur in the cytoplasm. To be clear, P-bodies are in the cytoplasm (ie they are not separated by a membrane). There is also dynamic ...
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3 votes

Small non-coding RNA (sRNA) vs. micro RNA (miRNA)?

Small non-coding RNAs are NOT generally abbreviated as sRNA but as sncRNA, if anything. I say "if anything" because the "small" part is subjective and just a portmanteau descriptor. The straight ...
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2 votes
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Why do RNAi pathway proteins exist in the nematode when they can cause them harm?

There are many studies that have used RNAi against the plant parasite M. incognita. The fundamental idea is that RNAi is directed against one of the vital genes of this nematode. Since, RNAi causes ...
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2 votes
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Small non-coding RNA (sRNA) vs. micro RNA (miRNA)?

miRNA are one member of the small non-coding RNA family. "Small non-coding" is a pretty broad term that encompasses microRNA and short interfering RNA, among other regulatory RNA species. The key ...
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2 votes

Are non-coding RNAs introns?

Yup - in a lot of cases the transcription of non-coding RNAs is from introns. Some of them are within genes (intragenic) and others are intergenic (between genes). In humans, a large number of both ...
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2 votes
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Can CRISPR knockout of a gene be referred gene inhibition?

Looking to a standard source of definitions, the Systems Biology Ontology definition of inhibition is "Negative modulation of the execution of a process." As such, it doesn't really make ...
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1 vote
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Where in the cell does this enhancer RNA knockdown take place?

RNAi can happen in the nucleus as well. This is better documented in C.elegans but there are some references supporting nuclear RNAi in mammalian cells too: https://doi.org/10.1073/pnas.1707440114 ...
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1 vote

RNA interference and the former Petunias experiment >

They introduced a foreign gene responsible for petunia leaf colour, with the intentions of up-regulating gene expression for this colour, which was purple. What was observed however was intriguing. ...
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1 vote
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Microinjection method in C. elegans

What they're doing is called two-step PCR. In step one, some gene-specific primers with tags amplify genomic DNA, but the product has adapter regions now added to each end. In the second step, you add ...
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1 vote
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RNAi in nematode resistant plants

The question is little bit unclear: is in vitro or in vivo introduction in question? In vivo: As the nematode feeds on the plant during its parasitic phase, it consequently assures the ...
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1 vote

Why introduction of an extra copy of a gene related to pigmentation causes RNA interference in Petunia?

The answer is in the slides you provided a link to. The key fact is that Craig & Andy did careful controls to show that it was the presence of dsRNA in both the sense control, and in the anti-...
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