Stack Exchange Network

Stack Exchange network consists of 175 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Determining the sequence of RNA. This technique is used for discovery and quantification of mRNAs and ncRNAs.

Involves library preparation and sequencing. Library preparation might slightly vary for different classes of RNAs. The general procedure for mRNA and most ncRNA sequencing involves:

  1. Enrichment of polyA RNAs using oligo-dT beads to remove ribosomal RNA. Alternatively, ribosomal RNAs can be selectively removed. Refer to this.
  2. cDNA preparation
  3. Adapter ligation
  4. PCR amplification
  5. Sequencing

See also .

history | excerpt history