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General Considerations The question asks specifically why certain plant products are not produced commercially in genetically modified micro-organisms. There are some general reasons, illustrated in some of the examples mentioned: The product is actually a complex mixture, rather than a single compound. This is the case for saffron, preparations of which ...


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I'm not well-verse in fungal transcriptional system but I did some digging in bacteria before, so not entirely sure this perspective will help. The promoter region of any gene should be upstream of the transcriptional unit and usually remains in the intergenic region, not being part of the CDS of interest and the adjacent gene. If we're digging a promoter of ...


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Why does the same promoter within the same organism have such varied sequences? While there may be other reasons, the fact that the same species has several genes encoding GPD proteins is an indication that this enzymatic activity is important for different mechanisms, times or conditions. For example, I mentioned in a comment to one of your previous ...


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Your strategy would work, but if possible you might be better off using XmaI rather than SmaI, as the latter is a blunt-end cutting enzyme. Furthermore, your strategy would not replace the entire gpdA promoter, if that's what you're after. You might wanna refer to their paper, where it's clear from Fig. 1 that the promoter region is much longer than the ...


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