2
votes
293T safety, transfections with plasmid DNA
293T cells do incorporate partial virus genomes, consisting of about 4000 bases (about 11%, if I remember right) of the adenovirus type 5 genome (Cloning and Sequencing of the Cellular–Viral Junctions ...
- 14.3k
2
votes
Accepted
In plasmid-based transient transfection of mesenchymal stem cells, do I have to select for transfected cells and verify GOI expression via a reporter?
No and Yes, respectively.
No, you don't need to select for and generally you can't unless you use something like FACS to sort and capture cells expressing your marker/GOI.
Transient transfections are ...
- 12.9k
1
vote
293T safety, transfections with plasmid DNA
Washington State has a nice risk assessment out of their biosafety department that outlines the risks associated with 293 cell lines (downloads a docx). The risk in itself is predicated upon poor work ...
- 8,119
1
vote
Luciferase promoter vector over p-AcGFP1-C1 vector
You are confusing luminescence and fluorescence. GFP does not emit light. The abbreviation stands for Green Fluorescent Protein. You need to shine a blue light source at it and it will fluoresce green....
- 1,425
Only top scored, non community-wiki answers of a minimum length are eligible