9
votes
Accepted
Is hydrochloric acid suitable for pickling food?
Short answer
Weak organic acids are more effective for food preservation then strong mineral acids, mainly because undissociated weak acids can cross the cell membrane and disrupt cell physiology from ...
- 51.8k
9
votes
Accepted
Evolution home experiment?
Keep in mind that the citrate utilizing strain appeared after over a decade. Also, Escherichia coli could always metabolize citrate, but the citrate transporter that brings it into the cell is only ...
- 17.6k
8
votes
Accepted
Meaning of "f.a." in the species name
"Forma asexualis" as explained in another paper
The authors favor the use of the expression forma asexualis (f.a.) in the
description of anamorphic species of the genus
Cystobasidium and this ...
- 3,042
5
votes
How does DNA damage cause ageing in yeast?
In eukaryotic cells there is no difference between a mother and a daughter cell - the later is an exact copy of the mother cell. This is true for yeasts as well for example for human cells. The only ...
- 51.1k
4
votes
Earliest to use media after autoclaving?
There is a relatively simple (and yet logical) answer: You can use the media as soon as they are cold enough for your desired culture temperature. So if you want to use the media at 30°C, there is no ...
- 51.1k
4
votes
How does DNA damage cause ageing in yeast?
Background to the different theories of ageing.
This video, from a senior lecturer at the University of Liverpool who specialises in ageing, discusses the theories of ageing. He touches on the DNA ...
- 11.3k
4
votes
Accepted
How can I measure the population growth of yeast?
The standard way to measure growth in a liquid culture is to measure the optical density (OD) of the solution – basically, how cloudy it is. Bacteria or yeast in a solution will absorb light that ...
- 345
3
votes
Accepted
Why does yeast react the way it does to water combined with sugar?
Yeasts are living organisms, they do not evaporate. However it is true that in most fermented food we eat, yeast are no longer there. So why is it so?
It is not the reaction with sugar and warm water ...
- 677
3
votes
Where do kombucha SCOBYs get nitrogen?
Short answer:
Nitrogen fixation (i.e, atmospheric nitrogen)
Longer answer:
Nitrogen fixation is the process by which atmospheric nitrogen is converted to forms usable by further biochemical ...
- 40.1k
3
votes
Accepted
Can yeast survive without mitochondria?
Basic answer: Yes, they can survive, but only if they do not need to undergo cellular respiration. I quote from Microbiological Research, Volume 169, Issue 2-3, p. 185-195:
Mitochondrial DNA (...
- 2,721
3
votes
Is the CMV promoter active in yeast?
Yes (reference)
While I found your question interesting this was pretty much the first hit when I typed "CMV yeast" in google scholar. You should really look there first before you ask!
- 1,415
3
votes
Enzyme Inhibitors: Using in vivo in yeast
This is usually the way this kind of inhibitors are used. There needs to be a way that they come into the cells (if they stay in solution and cannot reach their target they will be useless), but then ...
- 51.1k
3
votes
Accepted
Relationship between turgor pressure and osmotic pressure?
Turgor Pressure(T.P) is defined as the amount of pressure inside a cell solely due to its water content, whereas Osmotic Pressure(Pi) is defined as the pressure required to stop osmotic flow between ...
3
votes
Accepted
Can yeast in a primary fermentation of wine be used as a source of yeast for new fermentation?
Yes, it is possible to reuse yeast in both beer and wine fermentation - commercial brewers do it all the time for cost savings and batch reproducibility, and although I'm not as familiar with making ...
- 15.2k
3
votes
How can I estimate the total number of yeast cell in a medium?
Determination of cell concentration using a microscope is usually done by using a hemocytometer, also called counting chamber or Neubauer-counting chamber.
Basically, you count the cells in a defined ...
- 141
3
votes
Accepted
Can viruses stabilize and increase the shelf life of wine and other foods?
There is at least one bacteriophage ("phage") that targets bacteria that spoil wine:
The Gluconobacter phage GC1 is a novel member of the Tectiviridae family isolated from a juice sample collected ...
- 5,550
3
votes
Accepted
What is a "marker-matched" plasmid?
It is where a control plasmid is used - this is usually an empty vector or a non-target vector with the same "marker" on the plasmid.
The marker is often one that is either used for ...
- 9,024
2
votes
Sources for common laboratory Saccharomyces strains?
One very important resource is EUROSCARF. http://web.uni-frankfurt.de/fb15/mikro/euroscarf/
It is one of the very famous and dedicated strain repository for yeast (S. cerevisiae) strains. You can ...
- 261
2
votes
Yeast absorption of different spectra of light
Most of the loss of light being transmitted through individual cells is not absorbed, rather it is scattered (redirected from its original direction to a new one without loss of energy). There is ...
- 912
2
votes
Accepted
The effects of auto-brewery syndrome
The gut microbiome is extremely complicated, and almost anything related to it is only partially known, therefore prone to oversimplification. Trying to explain the phenomenon of gut fermentation ...
- 24.5k
2
votes
Earliest to use media after autoclaving?
If not using antibiotics or other additives, it's just a matter of medium being cold enough. If it's too hot, the yeast (or whatever you are inoculating) might get killed or heat-shocked, which you ...
- 4,470
2
votes
Accepted
Yeast Strain with squalene synthase / farnesyltransferase (ERG9) gene deleted
Yeast strains have two mating types MATa (since it secretes a pheromone named 'a factor'), MATα (secretes pheromone 'α factor'). Both MATa and MATα strains are haploid and when they fuse together they ...
- 391
2
votes
Accepted
Rice left in water for several months - what biological reactions or growths would occur?
It is likely a volatile organic compound (VOC) that is released as a by-product of microbial metabolism, although microbes can also produce some inorganic compounds, such as hydrogen sulfide (rotten ...
- 258
1
vote
Can a plasmid cause cancer?
Canonically, bacterial plasmids will not replicate in eukaryotic systems, due to a lack of the necessary control (regulatory) elements which will lead to expression.
It is possible though to ...
- 6,124
1
vote
How to measure the length of mitochondria from z stack fluorescent microscopy image?
ImageJ is usually the standard software to measure cell characteristics, a little bit of a learning curve but there is a large suite of analysis methods and image adjustments/filters.
- 321
1
vote
Accepted
Yeast contamination with fungus patch
Either you can cut out the fungus and get away with it, or the fungus will rapidly overgrow your plate regardless. You might as well try to cut out the fungus. If it overgrows your plate the ...
- 2,964
1
vote
How do I improve expression using the GAL1 promoter in S. cerevisiae above the "leaky" level I'm currently seeing?
There are a couple of things that are strange here. First, you have low expression in the presence of galactose. The GAL1 promoter is really strong, so that strikes me as odd. Even if it was leaky, ...
- 176
1
vote
Pichia pastoris and ornithine
Yes, it contains arginase. Thise enzyme converts arginine to ornithine and is probably part of the urea cycle.
You might notice the arginase is listed as coming from Komagataella phaffii GS115, but ...
- 1,463
1
vote
Using PGAL10 promoter to overexpress specific yeast genes: How to?
You're right about them being promoters, although it would be useful if you posted the link to the source of the sentence that you cite, so that we can read exactly how they are being used.
A fairly ...
- 1,879
1
vote
What concentration of sugar is optimal for baker's yeast fermentation?
When glucose and fructose are fermented separately, the uptake profiles indicate that both sugars are utilized at similar rates. However, when fermentations are conducted in media containing an equal ...
- 172
Only top scored, non community-wiki answers of a minimum length are eligible