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Yeast grows very well in liquid. The initial innoculum from the store will visible grow (“prove”) in warm water with a little sugar.


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To predict the number integrations is probably not possible, but you can favor multiple integrations, if that's what you are after. At least in Saccharomyces cerevisiae integration of a first plasmid appears to stimulate the insertion of additional plasmids at the same site (1). It is therefore possible to favor multiple integrations (2) by increasing the ...


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There absolutly is selection involved. First, you create a selective environment to culture a subset of environmental organisms (yeasts). Then, by infusing fresh media (flour/water) into the cultures each day, you are essentially passaging a live culture or organisms. It's not rational selection, like that practiced by plant breeders, since you aren't hand ...


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Yes, continually diluting the mix like this encourages the organisms (bacteria, yeasts, fungi) to have more constant conditions (maximum nutrients, fewer waste products) and be in log phase growth. The mix selects for particular faster-growing organisms. if you did not dilute, the nutrient levels would change (lower) and waste products accumulate much faster....


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