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CuriousTree

Knows a bit about proteins, enzymes, structural biology, biotechnology, microbiology and molecular biology...but always attempting to understand more chemistry...

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  • Member for 6 years, 2 months
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  • Last seen Mar 6 at 16:34

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8 Optimal pH of protein buffer? Basic principles to adjust buffers according method and analysis Jul 8 '16
6 What is the fastest way to crystallise lysozyme (for student course)? Feb 25 '19
6 Non-ribosomal peptide synthesis: why Glutathione cannot be produced by the ribosome? Oct 31 '15
4 Problems with SDS-PAGE, no separation - what could be the problem? Mar 3 '17
3 Clarify pre-column pressure vs. system pressure vs. backpressure for prepacked columns used with the äkta purification system Dec 30 '16
3 What is the shortest mRNA the ribosome can read to produce a peptide? Nov 2 '15
3 When is strict sterile technique necessary? Cloning vs. protein expression Jan 13 '17
3 Measuring protein concentration, Bradford vs. Nanodrop? Jan 16 '17
3 Protein purification: Linear elutions or stepwise elution, using imidazole or pH gradient? Jan 14 '17
3 Why is sorbitol used in buffers? Sep 4 '20
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