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CuriousTree
  • Member for 9 years, 2 months
  • Last seen more than a month ago
  • Norway
8 votes
1 answer
552 views

Optimal pH of protein buffer? Basic principles to adjust buffers according method and analysis

8 votes
0 answers
171 views

What is the fastest way to crystallise lysozyme (for student course)?

6 votes
2 answers
825 views

Non-ribosomal peptide synthesis: why Glutathione cannot be produced by the ribosome?

5 votes
4 answers
10k views

Measuring protein concentration, Bradford vs. Nanodrop?

4 votes
1 answer
1k views

Problems with SDS-PAGE, no separation - what could be the problem?

3 votes
1 answer
863 views

What is the shortest mRNA the ribosome can read to produce a peptide?

3 votes
1 answer
7k views

Clarify pre-column pressure vs. system pressure vs. backpressure for prepacked columns used with the äkta purification system

3 votes
2 answers
1k views

When is strict sterile technique necessary? Cloning vs. protein expression

3 votes
1 answer
1k views

Protein purification: Linear elutions or stepwise elution, using imidazole or pH gradient?

2 votes
2 answers
2k views

How to build a trimeric protein structure from monomeric PDB file?

2 votes
1 answer
4k views

Optimizing Gel Electrophoresis: Ampere, Volts and Buffer concentrations

2 votes
1 answer
129 views

When in Ampicillin degraded (gone) in liquid TB-media? Concerns about selectivity

2 votes
1 answer
760 views

Programs/software to graph biological data [closed]

2 votes
0 answers
281 views

Does glycerol in E.coli culture media somehow inhibit the lac-operon?

1 vote
1 answer
114 views

Quantification of various amino acids from bacteria?

1 vote
1 answer
336 views

Enzyme kinetics: recommended literature to grasp the concepts better

1 vote
1 answer
4k views

PBST vs. TBST buffer in western blotting