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CuriousTree
  • Member for 7 years, 6 months
  • Last seen more than a month ago
  • Norway
8 votes
1 answer
514 views

Optimal pH of protein buffer? Basic principles to adjust buffers according method and analysis

8 votes
0 answers
88 views

What is the fastest way to crystallise lysozyme (for student course)?

6 votes
2 answers
744 views

Non-ribosomal peptide synthesis: why Glutathione cannot be produced by the ribosome?

5 votes
4 answers
6k views

Measuring protein concentration, Bradford vs. Nanodrop?

4 votes
1 answer
942 views

Problems with SDS-PAGE, no separation - what could be the problem?

3 votes
1 answer
710 views

What is the shortest mRNA the ribosome can read to produce a peptide?

3 votes
1 answer
5k views

Clarify pre-column pressure vs. system pressure vs. backpressure for prepacked columns used with the äkta purification system

3 votes
2 answers
1k views

When is strict sterile technique necessary? Cloning vs. protein expression

3 votes
1 answer
804 views

Protein purification: Linear elutions or stepwise elution, using imidazole or pH gradient?

2 votes
2 answers
2k views

How to build a trimeric protein structure from monomeric PDB file?

2 votes
1 answer
3k views

Optimizing Gel Electrophoresis: Ampere, Volts and Buffer concentrations

2 votes
1 answer
108 views

When in Ampicillin degraded (gone) in liquid TB-media? Concerns about selectivity

2 votes
1 answer
677 views

Programs/software to graph biological data [closed]

2 votes
0 answers
112 views

Does glycerol in E.coli culture media somehow inhibit the lac-operon?

1 vote
1 answer
96 views

Quantification of various amino acids from bacteria?

1 vote
1 answer
267 views

Enzyme kinetics: recommended literature to grasp the concepts better

1 vote
1 answer
3k views

PBST vs. TBST buffer in western blotting