Thomas Ingalls
  • Member for 10 years, 1 month
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Do men have more extreme variations than women?
6 votes

The biggest problem with this question, (not just here, but for everyone who asks it,) is drawing on our culturally constructed definition of maleness to look for mechanisms of genetic inheritance of ...

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What is meant in biology by the term "evolved"?
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26 votes

"More evolved" is actually meaningless in all contexts. See terdon's answer for a good explanation. In the strictest sense, an organism can be said to be more divergent than another when comparing ...

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Bacterial cell lysis buffer used in proteomics procedures
0 votes

There are a few techniques available for this. Some recommend a urea-based technique, others recommend a chloroform. Do you have more information about your specific needs? A Chloroform-Assisted ...

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Is there a limit to acceptable brightness for plants?
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4 votes

The wikipedia page on photosynthetic efficiency has some useful insight here: Photosynthesis increases linearly with light intensity at low intensity, but at higher intensity this is no longer the ...

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Why aren't mitochondria and plastids considered symbiotes of eukaryotic cells?
7 votes

I would say it has to do with the amount of mitochondrial or sequence that has been transferred to the host genome. As a consequence of all this information stored in the host genome, mitochondria ...

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Can any protein be phosphorylated?
7 votes

Phosphorylation requires exposed serine, threonine, tyrosine, or histidine residues (in eukaryotes). This is because the transfer of phosphate groups to proteins is mediated by a class of proteins ...

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Can DNA produce ribozyme-like structures?
4 votes

DNA has recently been designed to act as a targeted drug-delivery agent at George Church's lab. This doesn't involve catalyzing a reaction between two substrates, but it does involve significant ...

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How to set up a slow cooling on an AB Veriti thermal cycler?
4 votes

If I recall correctly (from when I interned at ABI), you can't directly set this in the Veriti interface. However, (again, I'm not 100% certain -- I couldn't find anything to confirm this after 30 ...

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How does the enzyme ATP Synthase use a proton concentration gradient to make ATP?
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10 votes

Image from wikipedia page on ATP synthase In brief, the addition and release of protons to the structure cause a conformational change that leads to another conformational change. This series of ...

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How do I change bond colors to something besides a different hue in Coot?
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5 votes

You could try other visual differences, like make what you're interested in ball-and-stick, and everything else wireframe, like in this example: . .... Because, from: http://www.ysbl.york.ac.uk/~...

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How is Taq polymerase produced?
6 votes

For recombinant Taq polymerase, industrial-scale production produces liters of highly concentrated enzyme in a single run. It comes in such small, dilute quantities when sold that only a few preps a ...

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Online Molecular and Cellular Biology Video Lectures?
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8 votes

MIT's OCW is weak when it comes to biology videos. UC Berkeley has some good video content for molecular and cell biology. See, for example, this playlist: http://www.youtube.com/playlist?list=...

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Alternate genetic codes in newly sequenced organisms
2 votes

The short answer is yes, you could end up with many errors. See here for more detail: NCBI: "The Genetic Codes"

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How many times did endosymbiosis occur?
16 votes

I think @mbq has covered the frequency question better than I can. There is at least one modern example of this kind of new organelle formation. Aphids have a deep, intracellular endosymbiont ...

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