A. Radek Martinez
  • Member for 6 years
  • Last seen more than a month ago
  • Toronto, ON, Canada
Can the central dogma work in reverse?
4 votes

Contrary to my belief, it may be possible. The main concern we must look at is Protein to RNA, as we know RNA to DNA can be done through reverse transcription. Some evolutionary scientist believe ...

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When in Ampicillin degraded (gone) in liquid TB-media? Concerns about selectivity
3 votes

rather interesting question. Selection pressure is very important for any type of work. And it is true, amplicilin will degrade in media due to the secretion of beta-lactumase. This is a limitation ...

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Fine permanent fluorescent marker for blue light
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3 votes

I have had this problem before... this is what I ended up using: https://www.amazon.com/Invisible-Blacklight-Marker-Blue-Yellow/dp/B004C89M9Q You may have to write on a tape or something so that the ...

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What is a Slurry in Microbiology?
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2 votes

Apparently, microbiologist use this term to describe the process of culturing microorganisms from soil. You make a 'slurry' which has both growth media and the soil (which would fall under the english ...

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What is a subgenomic promotor?
1 votes

A subgenomic promoter is a promoter added to a virus for a specific heterologous gene, resulting in the formation of mRNA for that gene alone. Many positive-sense RNA viruses produce these subgenomic ...

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Is it safe to autoclave seawater agar?
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1 votes

I see no reason why it would precipitate chemically. Besides, you need to dissolve the agar via heat. Hence, auclaving is fine. If you do not believe me these protocol allow for autoclaving of ...

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Why do I have two kinds of pubic hair?
1 votes

Relatively interesting evolution question --- hypothesis regarding reasons for certain evolutionary traits can be debated (this particular question, scientists seem to have many theories). However, ...

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Why should you use an annealing temperature about 5°C below the Tm of your primers?
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1 votes

The annealing temperature of a primer is defined as the temperature in which 50% of the nucleotides of the primer are bound to the DNA. 50% annealing proportion will not yield an optimal PCT product. ...

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How to feed bacteria out of lab
1 votes

In a lab there are so many ways of doing this. However, at home is a little tricky. This is what I would do: Keep your bacteria in a liquid media. Liquid Media: make the media out of NaCl and AS ...

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Full Sequence for Plasmid pMG101
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1 votes

Here it is: http://www.ncbi.nlm.nih.gov/nuccore/4206623?report=graph This is the same plasmid as in E. Coli J53. This is the newest sequence.

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DNA denaturation and Renaturation
0 votes

It really depends on a variety of factors. (1) the nature of the dsDNA, is it a plasmid or chromosomal DNA? Plasmids more easily renature than chromosomes, so rapid cooling and heating my not affect ...

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Is protein folding symmetric with respect to reversing the sequence order?
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0 votes

No! Although there is a relationship, the protein would not fold properly since the C and N terminals are reversed consider the following: H(NH)-A-C(=O)(NH)-B-C(=O)(NH)-C-C(=O)(NH)-D-C(=O)(NH)-E-(C=...

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Dealing with Repetitive Bases in DNA?
0 votes

Okay, one of the main problems with polA tails is with PCR, which is a method commonly used in molecular cloning, specified mutagenesis etc... One problem with PolyA tails and PCR is with the primers....

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Is Plasmid pGLO a stable or unstable plasmid varient ?
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0 votes

The pGLO plasmid uses a stable version of GFP. This is stated in the pGLO education series protocol, and wiki. I could however, engineer to be unstable if wanted. https://en.wikipedia.org/wiki/PGLO

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Performing PCR on samples whose DNA concentration is nul
0 votes

Some times nanodrop is unreliable. However, to be sure that you are not wasting your time, I would redo the extraction. Nanodrop in a lot of PCR methods is a control test to validate that the ...

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Good microscope?
0 votes

So there are a couple of things you should ask yourself: a) how 'small' do you want to see b) what are you trying to study - (microspores can differ depending on that. Examples: Microbiology - (...

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