User Mad Scientist

21 votes

1 answer

1k views

Can siRNA induce DNA methylation in mammalian cells?

asked Dec 16, 2011 at 9:00

17 votes

4 answers

7k views

What implications has the missing 2'-OH on the capability of DNA to form 3D structures?

asked Dec 22, 2011 at 13:21

15 votes

2 answers

4k views

Are there issues with filling PCR tubes to capacity?

asked May 21, 2014 at 12:06

14 votes

1 answer

28k views

Why does RNA adopt an A-form helix?

asked Feb 25, 2012 at 11:17

12 votes

2 answers

60k views

Why is SOC medium recommended for transformations?

asked Mar 26, 2012 at 8:55

12 votes

1 answer

583 views

Which sequence characteristics influence the transcription efficiency of T7 polymerase?

asked Dec 15, 2011 at 8:36

12 votes

2 answers

16k views

Why should I degas my gel solution for polyacrylamide gels?

asked Feb 9, 2012 at 9:42

11 votes

1 answer

2k views

Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?

asked Mar 29, 2012 at 13:58

11 votes

2 answers

12k views

What is the advantage of using starter cultures for growing bacteria?

asked Mar 28, 2012 at 10:45

8 votes

4 answers

240 views

How can I produce milligram quantities of an isotope-labeled DNA oligomer?

asked Dec 15, 2011 at 20:11

8 votes

2 answers

2k views

Alternatives to ethidium bromide for staining small nucleic acids?

asked Jan 3, 2012 at 15:35

8 votes

1 answer

8k views

How do I read a sequence logo?

asked Jul 31, 2014 at 15:27

8 votes

2 answers

21k views

How do I deal with sticky and viscous samples from cell lysates?

asked Jun 28, 2012 at 15:05

7 votes

1 answer

1k views

Determining if a specific proline is cis or trans in the protein?

asked Nov 9, 2012 at 9:23

7 votes

1 answer

134 views

What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?

asked Nov 20, 2012 at 11:47

7 votes

2 answers

17k views

How can I resuspend a cell pellet without harming the bacteria?

asked Mar 4, 2013 at 8:04

7 votes

1 answer

115 views

Which factors besides the thermodynamic stability are important for the hairpin in intrinsic transcription termination?

asked Mar 21, 2012 at 10:09

6 votes

2 answers

2k views

For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?

asked Mar 29, 2012 at 13:10

6 votes

3 answers

2k views

How useful are vitamins and trace elements in E. coli culture medium?

asked Oct 29, 2012 at 9:30

5 votes

2 answers

600 views

How can I fetch nucleotide sequences based on ENA accession numbers and positions?

asked Feb 13, 2014 at 16:01

3 votes

1 answer

1k views

Why are riboswitches mostly present in bacteria and not in eukaryotes?

asked Jan 22, 2015 at 19:28

1 vote

0 answers

24 views

Can you gain valid toxicological data about a detergent in cell cultures?

asked Aug 11, 2018 at 18:20

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22 Questions

Can siRNA induce DNA methylation in mammalian cells?

What implications has the missing 2'-OH on the capability of DNA to form 3D structures?

Are there issues with filling PCR tubes to capacity?

Why does RNA adopt an A-form helix?

Why is SOC medium recommended for transformations?

Which sequence characteristics influence the transcription efficiency of T7 polymerase?

Why should I degas my gel solution for polyacrylamide gels?

Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?

What is the advantage of using starter cultures for growing bacteria?

How can I produce milligram quantities of an isotope-labeled DNA oligomer?

Alternatives to ethidium bromide for staining small nucleic acids?

How do I read a sequence logo?

How do I deal with sticky and viscous samples from cell lysates?

Determining if a specific proline is cis or trans in the protein?

What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?

How can I resuspend a cell pellet without harming the bacteria?

Which factors besides the thermodynamic stability are important for the hairpin in intrinsic transcription termination?

For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?

How useful are vitamins and trace elements in E. coli culture medium?

How can I fetch nucleotide sequences based on ENA accession numbers and positions?

Why are riboswitches mostly present in bacteria and not in eukaryotes?

Can you gain valid toxicological data about a detergent in cell cultures?