Timeline for What is the process by which fluorescent proteins in two photon microscopy are optically stimulated by membrane depolarization?
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| Jul 14, 2021 at 3:56 | comment | added | Bryan Krause♦ | @C-Consciousness The wikipedia page I linked on two photon microscopy explains it fairly well. It's not really anything special about biology, it's a chemistry/physics thing about excited states. Two long wavelength photons can have just as much energy as one shorter wavelength photon so it excites the molecule to the same state from which it then emits a photon is the short story. | |
| Jul 14, 2021 at 3:21 | comment | added | C-Consciousness | Thank you for your answer. This is more of a question related to the fluorophore. As I understand, green fluorescent protein transform ultraviolet-blue light to green light, however I am unclear how the protein do the non-linear photon conversion of infrared light. Could you expand on that? Thank you | |
| Jul 12, 2021 at 20:31 | history | answered | Bryan Krause♦ | CC BY-SA 4.0 |