Timeline for Does an ORF need a stop codon?
Current License: CC BY-SA 4.0
7 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| Sep 28, 2022 at 19:22 | comment | added | Daniel Van Antwerp | Also, remember that instructors' favorite trick question is find the ORF in an mRNA sequence: A) augccg....stop or B) atgccg...stop...Tricky...tricky | |
| Sep 28, 2022 at 19:20 | comment | added | Daniel Van Antwerp | Before I definitively say yes, tell me the size of the sequences you are being given to find an ORF. If they are really short, your instructor may just be trying to get you to find an ATG. But if they are long and some ATGs do have an in-frame stop codon and others don't, I'd say the instructor is wanting you to identify an actual ORF...ATG to STOP. | |
| Sep 28, 2022 at 18:27 | comment | added | yemy | Okay. ATG to STOP codon means that ATG without a following STOP codon is no ORF? | |
| Sep 28, 2022 at 18:04 | comment | added | Daniel Van Antwerp | Darn. I hate it when biology gets confusing like this. Class assignments, depending on the level, can be almost too straightforward. Stick with the straight answer...ATG to STOP codon to find your ORFs. The Web.expasy.org/translate app is pretty weak and just meant to take sequence and find ATGs. Just like I mentioned before...futile. You could get the same answer by taking the sequence into MS Word and using the Find function to search for ATG. The only thing the app gives you is all the additional translation which doesn't mean anything if it's not a real ORF. | |
| Sep 28, 2022 at 16:59 | comment | added | yemy | It's actually for an assignment and the task is to read ORFs from DNA. In the assignment every start with stop codon is an ORF. With your answer I am just more unsure yet. Websites like web.expasy.org/translate also count a dna with no stop codon as ORF. | |
| S Sep 28, 2022 at 16:56 | review | First answers | |||
| Sep 30, 2022 at 9:42 | |||||
| S Sep 28, 2022 at 16:56 | history | answered | Daniel Van Antwerp | CC BY-SA 4.0 |