Timeline for How do molecular biologists typically get their oligos?
Current License: CC BY-SA 4.0
6 events
| when toggle format | what | by | license | comment | |
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| Oct 2, 2023 at 17:44 | comment | added | Maximilian Press | @MikeyC i think that your comment is a different (and possibly more useful) answer from mine if you want to post it there | |
| Oct 2, 2023 at 17:22 | comment | added | Gumpf | I see, is the same true for RNAs or would you just order a template DNA and run in vitro transcription? | |
| Oct 2, 2023 at 17:09 | vote | accept | Gumpf | ||
| Oct 2, 2023 at 17:01 | comment | added | MikeyC | Depends on your application. For smaller to medium sized DNA oligos (<~120 bp) or medium sized dsDNA fragments (<3kb), I will always order them. What you're describing sounds a bit like Gibson assembly cloning (en.wikipedia.org/wiki/Gibson_assembly), and I would only go that route for fragments upwards of ~3kb, unless I needed to make several of them at once (also assuming I know the full sequence needed). Larger fragments can be purchased, but the price per bp can get high for very long fragments (still might be worth it for a one-off). | |
| Oct 2, 2023 at 16:48 | answer | added | Maximilian Press | timeline score: 2 | |
| Oct 2, 2023 at 15:27 | history | asked | Gumpf | CC BY-SA 4.0 |