Timeline for How come SSBPs in RPA don't bind primers?
Current License: CC BY-SA 4.0
5 events
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| Nov 22, 2023 at 21:17 | comment | added | David | @AndrewRoots — If you found part of my answer helpful you should consider upvoting or accepting it. As regards your opinion that my edits weren't necessary, I provide an explanation of the reasons for such edits on Meta. It is, of course, your prerogative to disregard them, and I do not wish to discuss this matter further. | |
| Nov 22, 2023 at 10:39 | comment | added | Andrew Roots | Thanks for the reply. Yes, I read that article, but it doesn't actually say that the SSBPs are added at a later stage. They only show up in panel D of the figure because that's where they're relevant, not because that's where they're added. Like the DNA polymerase is not added after the SSBP (panel E). Also, the manuals for commercial RPA kits do not specify that SSBPs are added after the primers, recombinase and template. However, your "less than 35nt" theory does make sense! And thanks for the edits to my question, but they weren't necessary. | |
| Nov 13, 2023 at 13:10 | history | edited | David | CC BY-SA 4.0 |
Added an illustration for clarification. Minor edit to text.
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| Nov 12, 2023 at 22:46 | history | edited | David | CC BY-SA 4.0 |
Added another possibility
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| Nov 12, 2023 at 16:47 | history | answered | David | CC BY-SA 4.0 |