Timeline for What is the minimum Ct value that you would consider indicates no gene expression?
Current License: CC BY-SA 4.0
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| Apr 1 at 17:11 | comment | added | MikeyC | I find that reaction chemistry often determines the upper range of usable Ct values. With intercalating dyes (SYBR), Ct values >~32 can start to blend with NTCs, but a melt analysis can help you tell if it's a true signal or not. With fluorescent probes, I generally count any Ct with decent technical reps, but in principle, a well designed reaction with good amplification efficiency should have single-copy Ct values at or below ~38 cycles, making quantitative interpretation of higher Ct values dicey (especially if you haven't established the linear range of the assay). | |
| Mar 31 at 19:02 | vote | accept | aquaporin | ||
| Mar 28 at 16:38 | answer | added | Maximilian Press | timeline score: 4 | |
| Mar 28 at 11:36 | history | asked | aquaporin | CC BY-SA 4.0 |