Timeline for How can I be more accurate in my cell counting?
Current License: CC BY-SA 4.0
5 events
| when toggle format | what | by | license | comment | |
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| May 21 at 20:06 | comment | added | bob1 | Too high is also possible - more than about 200/large square is getting hard to keep track of in terms of which ones you have counted (that's what the small subdivisions are for), so then you should dilute your cells and reload. However, you can do this quicker and easier than it is for too low and account for it. Lower numbers are more of a problem for accuracy. | |
| May 21 at 15:06 | comment | added | MakM | Thank you! I think 1-5 are the most likely to be my issue. Would it cause an issue if the cell concentration is "too high"? I was counting FB cells that should have been around 2.1M/mL (which is why I diluted 1:10). | |
| May 21 at 15:01 | vote | accept | MakM | ||
| May 21 at 0:05 | comment | added | anongoodnurse | Part of my work in Molecular Biology was with human fibroblasts. This is a very helpful answer indeed. | |
| May 20 at 21:45 | history | answered | bob1 | CC BY-SA 4.0 |