Timeline for What's the difference between protein and DNA behaviour during agarose gel electrophoresis?
Current License: CC BY-SA 3.0
13 events
| when toggle format | what | by | license | comment | |
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| Jan 17, 2014 at 9:58 | comment | added | Mad Scientist | I'd recommend to simply focus it on DNA and ignore proteins. You should also find out what equipment is available, you need a gel electrophoresis chamber (and they are different for agarose and PAA), power supply, the gel material itself (+ APS and TEMED for PAA), buffer, loading dye, something to stain the DNA or protein, and in the case of DNA also a UV lamp. | |
| Jan 17, 2014 at 9:51 | answer | added | Mad Scientist | timeline score: 3 | |
| Jan 17, 2014 at 9:29 | comment | added | Dan Humphrey | @MadScientist I'm fairly new to this (I'm only a high school freshman) and so haven't done adequate background research on the subject. I was just asking this question to see if such a project fits the required criteria and thus actually conduct a research. I'll consider polyacrylamide, though it's way more expensive and harder to find (I doubt I'll have access to the school lab or that they even have it). | |
| Jan 17, 2014 at 7:11 | history | tweeted | twitter.com/#!/StackBiology/status/424076526221795328 | ||
| Jan 16, 2014 at 21:16 | comment | added | Mad Scientist | As already mentioned, you don't use agarose for proteins, they're pretty much exclusively run on polyacrylamide gels, typically as an SDS-PAGE. I've never seen anyone run an agarose gel of a protein. | |
| Jan 16, 2014 at 20:42 | comment | added | Chris♦ | Thats in interesting question. I think so too, that these are mostly not proteins, since most proteins are not colored. | |
| Jan 16, 2014 at 20:35 | comment | added | MattDMo | which food coloring dyes will you be using? Most of them are based on small molecules, not proteins. | |
| Jan 16, 2014 at 20:21 | comment | added | Dan Humphrey | @chris Excuse the typo. I meant that electrophoresis will be run on food coloring dyes... you know... which are made of proteins | |
| Jan 16, 2014 at 20:16 | review | First posts | |||
| Jan 17, 2014 at 1:00 | |||||
| Jan 16, 2014 at 20:12 | comment | added | Chris♦ | What do you mean by "separating proteins in dyes"? Another thing: While you can't separate Proteins nicely on agarose, you can use low percentage polyacrylamide gels to separate DNA. | |
| Jan 16, 2014 at 20:06 | comment | added | Dan Humphrey | @Chris One of the requirements of the project is to run several simultaneous experiments while changing one variable only (in this case, it's the protein/DNA). I'm separating proteins in dyes, which would explain the use of agarose. | |
| Jan 16, 2014 at 20:02 | comment | added | Chris♦ | Proteins are usually not run on agarose gels since the pores of the gel are too big to have a good separation. A 1% agarose gel has pores of around 150nm, poly acrylamide (which is usually used) have 3-6nm depending on the degree of polymerisation and gel strength. You will only use agarose gels for really high molecular weight proteins otherwise you won't see much separation of the sample. DNA samples have such a high molecular weight and can thus be separated on agarose gels. | |
| Jan 16, 2014 at 19:59 | history | asked | Dan Humphrey | CC BY-SA 3.0 |