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Chris
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In my opinion wearing gloves during cell culture is a good idea. It works both ways as a protection: For your cells since you are not loosing small danders from your hand and for you since you are not getting media or chemicals used in cell culture on your skin when you are uncautious.

I usually prefer nitrile gloves over latex, since nitrile gloves are ways more stable and pose no risk for allergies (I am not allergic, but minimizing risks is always a good idea). The downside of nitrile is, that they are a bit more expensive (thats the point where you need to argue with some coordinators about what to buy).

I never had problems with desinfectants and gloves, but this is my personal experience and probably not more than an anecdote. I desinfect my gloves, since I touch different things with them, but I usually don't spray everything which comes around with alcohol since this is usually pointless. To be useful, it needs to reach all points of a surface and also needs to stay there for a while. What I make sure is that all the stuff, which goes into the hood is either very clean or packaged sterile anyway.

After my experiences some data, this PDF has quite a lot of information. The list below is taken from the PDF:

Nitrile gloves:

  • Acetone - fair
  • Ethanol - excellent
  • Isobutyl - alcohol excellent
  • Isopropyl - alcohol excellent
  • Methanol - fair

Latex gloves:

  • Acetone - good
  • Ethanol - excellent
  • Isobutyl - alcohol poor
  • Isopropyl - alcohol excellent
  • Methanol - fair

In my opinion wearing gloves during cell culture is a good idea. It works both ways as a protection: For your cells since you are not loosing small danders from your hand and for you since you are not getting media or chemicals used in cell culture on your skin when you are uncautious.

I usually prefer nitrile gloves over latex, since nitrile gloves are ways more stable and pose no risk for allergies (I am not allergic, but minimizing risks is always a good idea). The downside of nitrile is, that they are a bit more expensive (thats the point where you need to argue with some coordinators about what to buy).

I never had problems with desinfectants and gloves, but this is my personal experience and probably not more than an anecdote. I desinfect my gloves, since I touch different things with them, but I usually don't spray everything which comes around with alcohol since this is usually pointless. To be useful, it needs to reach all points of a surface and also needs to stay there for a while. What I make sure is that all the stuff, which goes into the hood is either very clean or packaged sterile anyway.

In my opinion wearing gloves during cell culture is a good idea. It works both ways as a protection: For your cells since you are not loosing small danders from your hand and for you since you are not getting media or chemicals used in cell culture on your skin when you are uncautious.

I usually prefer nitrile gloves over latex, since nitrile gloves are ways more stable and pose no risk for allergies (I am not allergic, but minimizing risks is always a good idea). The downside of nitrile is, that they are a bit more expensive (thats the point where you need to argue with some coordinators about what to buy).

I never had problems with desinfectants and gloves, but this is my personal experience and probably not more than an anecdote. I desinfect my gloves, since I touch different things with them, but I usually don't spray everything which comes around with alcohol since this is usually pointless. To be useful, it needs to reach all points of a surface and also needs to stay there for a while. What I make sure is that all the stuff, which goes into the hood is either very clean or packaged sterile anyway.

After my experiences some data, this PDF has quite a lot of information. The list below is taken from the PDF:

Nitrile gloves:

  • Acetone - fair
  • Ethanol - excellent
  • Isobutyl - alcohol excellent
  • Isopropyl - alcohol excellent
  • Methanol - fair

Latex gloves:

  • Acetone - good
  • Ethanol - excellent
  • Isobutyl - alcohol poor
  • Isopropyl - alcohol excellent
  • Methanol - fair
Source Link
Chris
  • 52.7k
  • 13
  • 124
  • 181

In my opinion wearing gloves during cell culture is a good idea. It works both ways as a protection: For your cells since you are not loosing small danders from your hand and for you since you are not getting media or chemicals used in cell culture on your skin when you are uncautious.

I usually prefer nitrile gloves over latex, since nitrile gloves are ways more stable and pose no risk for allergies (I am not allergic, but minimizing risks is always a good idea). The downside of nitrile is, that they are a bit more expensive (thats the point where you need to argue with some coordinators about what to buy).

I never had problems with desinfectants and gloves, but this is my personal experience and probably not more than an anecdote. I desinfect my gloves, since I touch different things with them, but I usually don't spray everything which comes around with alcohol since this is usually pointless. To be useful, it needs to reach all points of a surface and also needs to stay there for a while. What I make sure is that all the stuff, which goes into the hood is either very clean or packaged sterile anyway.