Timeline for Do you have experience with PacBio?
Current License: CC BY-SA 3.0
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| Apr 4, 2014 at 14:14 | comment | added | skymningen | I have never done this calculation for a sample this small with PacBio. To keep it short, from one SMRTcell you would get 30x coverage of the E. coli genome (no fiction, they have done 3 cells and got 100x coverage). I guess this is a bit oversized for your short samples. Still, I would rather not do any more "virtual" calculations about this, you need to have someone you can talk to who understands your experiment and probably has seen more PacBio sequencing than I have. | |
| Apr 4, 2014 at 12:35 | comment | added | tatiana.aghova | I am planning to sequence mouse genes of MHC a TLRs (about 2000 bp). Can you get me a fictitious example about how much sample can I involved by certain covarage?? Thanks a lot! ;) | |
| Apr 4, 2014 at 12:07 | vote | accept | tatiana.aghova | ||
| Apr 4, 2014 at 9:50 | history | answered | skymningen | CC BY-SA 3.0 |