Timeline for Hoechst red and blue spectra - what does the red emission tell us?
Current License: CC BY-SA 3.0
12 events
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| Aug 20, 2014 at 18:00 | comment | added | Damian Kao | Looking at the hoechst red spectrum is a pretty common practice in flow cytometry (examining the side population for example). It's not some random claim I made. Anyways, I found the answer in a paper. I updated the main question. | |
| Aug 20, 2014 at 17:58 | history | edited | Damian Kao | CC BY-SA 3.0 |
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| Aug 20, 2014 at 11:44 | comment | added | Armatus | That's a problem but it doesn't warrant sniping the question down immediately like this. Give the asker some time to edit the question and explain where he made such a claim before putting him through a tedious reopen procedure. If he doesn't respond within the next days, close it down. | |
| Aug 19, 2014 at 17:48 | review | Close votes | |||
| Aug 20, 2014 at 13:17 | |||||
| Aug 19, 2014 at 17:32 | comment | added | Behzad Rowshanravan | This question lacks evidence for the main claim made! | |
| Aug 19, 2014 at 7:46 | history | edited | Alan Boyd | CC BY-SA 3.0 |
edited body; edited title
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| Aug 18, 2014 at 21:30 | comment | added | Behzad Rowshanravan | could you provide a link or reference about the function of the dye as "indicative of apoptosis, cellular complexity, or a proxy for cytoplasmic content". I'm quite interested! I know that some dyes such as CyTRAK orange that stains both nucleus and cytoplasm with cytoplasm being a lighter orange colour than the nucleus but the described hoechst 33342 property is new to me, so I would be interested in any links/references you could provide supporting your question! | |
| Aug 18, 2014 at 19:52 | comment | added | Damian Kao | I am talking about Hoechst 33342 specifically. | |
| Aug 18, 2014 at 19:52 | history | edited | Damian Kao | CC BY-SA 3.0 |
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| Aug 18, 2014 at 19:46 | history | tweeted | twitter.com/#!/StackBiology/status/501455215196139522 | ||
| Aug 18, 2014 at 17:06 | comment | added | Mad Scientist | There are several Höchst dyes, you might want to clarify which you are talking about. | |
| Aug 18, 2014 at 15:48 | history | asked | Damian Kao | CC BY-SA 3.0 |