Timeline for Modelling how much cadmium a cell will be able to chelate with phytochelatins
Current License: CC BY-SA 3.0
7 events
| when toggle format | what | by | license | comment | |
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| Sep 25, 2014 at 12:39 | vote | accept | cpumar | ||
| Aug 30, 2014 at 20:21 | history | edited | Alan Boyd | CC BY-SA 3.0 |
edited body
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| Aug 28, 2014 at 1:09 | history | edited | gravityassist | CC BY-SA 3.0 |
Added link to relevant paper
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| Aug 28, 2014 at 1:02 | comment | added | gravityassist | Michaelis-Menton is only relevant to a simplified system that only has explicitly two variables. It seems from the original posting that this is the case. The original poster appears to be only trying to observe chelation and not transport to vacoule which makes this method a valid option. You are correct that you can treat the pathway as individual reactions but those reactions you mention must be considered independently for the Michaelis-Menton approximation to work. Additionally, cooperativity can be identified by a sigmoidal curve as in the classic case of hemoglobin. | |
| Aug 27, 2014 at 4:41 | comment | added | user137 | would michailis-menton really apply here? phytochelatin is a polymer that chelates metal ions and gets transported to the vacoule. I guess you could treat it like 2 enzymatic reactions, with chelation as step 1, and transport to vacoule as step 2. But you might get complex cooperativity as more metal ions bind to the phytochelatin. | |
| Aug 27, 2014 at 2:25 | review | First posts | |||
| Aug 27, 2014 at 4:12 | |||||
| Aug 27, 2014 at 2:24 | history | answered | gravityassist | CC BY-SA 3.0 |