Timeline for What is the difference between second and third generation sequencing
Current License: CC BY-SA 3.0
15 events
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| Feb 11, 2017 at 18:43 | comment | added | KingBoomie | An increase in throughput to discern between different generations is not true for second vs third generation sequencing (PacBio has the advantage of generating long reads but at a throughput lower than that of the second-generation sequencers: bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-699 ) | |
| Sep 9, 2014 at 11:34 | vote | accept | dresden | ||
| Sep 8, 2014 at 16:33 | history | edited | Superbest | CC BY-SA 3.0 |
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| Sep 8, 2014 at 16:30 | comment | added | Superbest | @AlanBoyd I am regrettably not well informed about the history of sequencing technologies past the last decade. I think you have a good point, edits to my question are welcome. | |
| Sep 8, 2014 at 8:51 | comment | added | Alan Boyd | @har-wadim thanks, I hadn't thought of it like that. What increase in throughput constitutes a generation? | |
| Sep 7, 2014 at 19:45 | comment | added | alephreish | @AlanBoyd Strictly saying, it is not about precedence: the distinction between the first generation and the next generation is in terms of throughput. In this respect both Maxam-Gilbert and Sanger belong to the same class of methods. | |
| Sep 7, 2014 at 8:15 | comment | added | Alan Boyd | Ultimately we, as scientists, shouldn't get hung up on the exact use of this terminology since it is clearly now just part of marketing the latest advance in sequencing technology. | |
| Sep 7, 2014 at 8:12 | comment | added | Alan Boyd | In my opinion referring to Sanger sequencing as 'first generation' is historically incorrect. Maxam-Gilbert sequencing preceded it and produced lots of useful results - see Addendum in my answer here: biology.stackexchange.com/questions/17598/… | |
| Sep 7, 2014 at 7:41 | comment | added | Superbest | @5heikki I was actually unfamiliar with MinION. Does it count as third-gen? Feel free to edit my answer and add it (or submit your own). | |
| Sep 7, 2014 at 1:01 | comment | added | 5heikki | Oxford Nanopore shipped MinIONs to a some researchers a few weeks ago. | |
| Sep 7, 2014 at 0:37 | history | edited | Superbest | CC BY-SA 3.0 |
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| Sep 7, 2014 at 0:31 | history | edited | Superbest | CC BY-SA 3.0 |
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| Sep 7, 2014 at 0:20 | history | edited | Superbest | CC BY-SA 3.0 |
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| Sep 7, 2014 at 0:14 | history | edited | Superbest | CC BY-SA 3.0 |
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| Sep 7, 2014 at 0:08 | history | answered | Superbest | CC BY-SA 3.0 |