Timeline for How to prepare sample for SDS PAGE?
Current License: CC BY-SA 3.0
9 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| Nov 17, 2014 at 7:53 | vote | accept | kt123 | ||
| Nov 14, 2014 at 11:58 | answer | added | Chris♦ | timeline score: 7 | |
| Nov 14, 2014 at 9:47 | comment | added | kt123 | @chris, Can you please give me protocol for preparing sample buffer? Because in different protocols showing different composition. | |
| Nov 14, 2014 at 7:21 | comment | added | Chris♦ | Surely it will affect the gel - the concentration of the ions is lower than it should be, so your gel can run funny. | |
| Nov 14, 2014 at 6:14 | history | tweeted | twitter.com/#!/StackBiology/status/533140934867709952 | ||
| Nov 14, 2014 at 5:24 | comment | added | kt123 | @Chris. Yes, my final concentration become 0.5x. Is it effect on getting bands on SDS PAGE? | |
| Nov 13, 2014 at 20:05 | history | edited | MattDMo |
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| Nov 13, 2014 at 17:35 | comment | added | Chris♦ | Why do you dilute like this? If you do so, the final concentration of your sample buffer is 0.5x. For this dilution you need a 5x sample buffer. | |
| Nov 13, 2014 at 17:25 | history | asked | kt123 | CC BY-SA 3.0 |