Timeline for Why doesn't Sanger (fluorescent) DNA sequencing double count nucleotides?
Current License: CC BY-SA 3.0
6 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| Dec 26, 2014 at 0:16 | comment | added | canadianer | @Alzeon Statistically, that seems unlikely given that there is billions of DNA molecules in a sequencing reaction. | |
| Dec 25, 2014 at 23:36 | comment | added | Alzeon | how do we know if a certain nucleotide hasn't been accounted for? (e.g., what if a fragment that terminated at nucleotide #70 was not produced?) | |
| Dec 25, 2014 at 8:18 | comment | added | jwillis0720 | I would like to also add that this is precisely why we use a capillary tube. The small surface area makes it ideal for resolution of small things like nucleotides. That is the whole idea behind chromatography. Trying to separate things that may only be modestly different in size (or charge) to separate. The more resolution something has, the farther each of those peaks will be from each other, and the sharper each peak will be, i.e. a very sharp line instead of a broad peak. | |
| Dec 24, 2014 at 18:48 | vote | accept | Alzeon | ||
| Dec 24, 2014 at 18:46 | history | edited | canadianer | CC BY-SA 3.0 |
deleted 3 characters in body
|
| Dec 24, 2014 at 18:24 | history | answered | canadianer | CC BY-SA 3.0 |