Timeline for Primer Design with Primer-BLAST over specific site
Current License: CC BY-SA 3.0
3 events
| when toggle format | what | by | license | comment | |
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| Dec 21, 2015 at 12:41 | comment | added | Marco Vismara | If he had to sequence KRAS gene, he had to amplify fragments of a determined maximum lenght, depending of the platform used. Also, do not forget that many sequencers need some bases (20-40) to warm up, and some other to land. E.G. I want to sequence a fragment from bp 100 to 150 of a given gene: I will design primers starting from bp 50-70 and stop at about 170. Personally I design primers pairs for any coding exon of a gene. | |
| Nov 19, 2015 at 13:24 | history | edited | AMR | CC BY-SA 3.0 |
Formatting to improve readability.
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| Jul 22, 2015 at 5:28 | history | answered | 243 | CC BY-SA 3.0 |