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Sep 14, 2015 at 12:08 comment added WYSIWYG Note that in Sanger sequencing the ddNTPs are not added together in the same mix. If you omit one ddNTP then you won't be able to sequence that particular base in the DNA. Though you may be able to infer the 4th base from the other three, the confidence of the read would be low.
Sep 14, 2015 at 12:05 history edited WYSIWYG CC BY-SA 3.0
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Sep 14, 2015 at 10:58 history answered Ro Siv CC BY-SA 3.0