Timeline for Why can't we use plasmids to add genes to ourselves?
Current License: CC BY-SA 3.0
9 events
| when toggle format | what | by | license | comment | |
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| Jan 25, 2018 at 9:31 | review | Close votes | |||
| Jan 30, 2018 at 3:01 | |||||
| Jan 25, 2018 at 2:52 | answer | added | Alexander Satchie | timeline score: 2 | |
| Apr 13, 2017 at 12:48 | history | edited | CommunityBot |
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| Oct 2, 2015 at 2:13 | comment | added | AMR | In mammalian cells, Plasmids are not replicated, which is why in cell culture, you need the extra step of recombining the vector into the genome via homologous recombination. The issue is that this can be done in vitro to cells, but once you try doing it on an organism level there are many problems. One is that Adenovirus usually needs to be used to insert the vector. But adenovirus' capsid can only accommodate about 10kb of DNA. As most homology arms are at least 10kb, each, you cannot do targeted integration. You would need to rely on random integration, and you couldn't predict the effects. | |
| Oct 2, 2015 at 1:11 | history | tweeted | twitter.com/#!/StackBiology/status/649753636092907520 | ||
| Oct 1, 2015 at 21:15 | answer | added | Roland | timeline score: 7 | |
| Oct 1, 2015 at 21:09 | comment | added | JDługosz | That makes sense. So "some yeasts" is the exception, having first evolved a mechanism to allow importing of survival traits, possibly trading off resistance to viruses. | |
| Oct 1, 2015 at 21:05 | comment | added | MCM | IIRC humans have several mechanisms that digest extra-nuclear DNA. Unfortunately I don't have a reference handy, but I'm 95% sure any plasmid would just be destroyed. | |
| Oct 1, 2015 at 20:20 | history | asked | JDługosz | CC BY-SA 3.0 |