Timeline for Nucleoside analogs that cause mutation
Current License: CC BY-SA 3.0
6 events
| when toggle format | what | by | license | comment | |
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| Oct 13, 2015 at 20:12 | comment | added | mdperry | Re: BUdR (bromouridine deoxyribose--that is what you are referring to?) I am not familiar with all the details, but off the top of my head, you would not expect to find U in DNA, only in RNA (ordinarily). Since T pairs with A in DNA, it follows that U pairs with A in RNA. In RNA double-stranded regions rU can also form base pairs with rG, as long as the Br atom does not disrupt this U=G pairing I would naively assume that dU (and by extension Br-dU) can base pair with dG. A dU=dC base pair would be quite unusual since they are both smaller, pyrimidines. | |
| Oct 13, 2015 at 20:08 | comment | added | mdperry | @Theresa Re: fate of nucleoside analogs incorporated into DNA, I don't actually know how long the modified base persists, but I have read papers where one entire strand was labeled, and the analogs could be detected 10 or 20 cell divisions later. If I understand correctly, the 2-AP can form base pairs with either dT or dC in subsequent rounds of DNA replication. | |
| Oct 11, 2015 at 16:51 | vote | accept | Theresa | ||
| Oct 11, 2015 at 16:51 | comment | added | Theresa | And another question. This tortora text said that 5-bromouracil would pair with cytosine (if it's not being fixed by DNA pol) but what the Brock's book said it actually binds with guanine. Which one is correct? Or 5-bromouracil actually capable of pairing with both of them? | |
| Oct 11, 2015 at 16:48 | comment | added | Theresa | Oh!! @mdperry, I've got it!!!! Thank you so much!! And I've 2 questions. I'm wondering if that cytosine form a complementary pair with guanine, then what about 2-aminopurine, since it's a semi conservative replication, we shouldn't leave 2-aminopurine hanging there correct? Would it pair with a new cytosine again? | |
| Oct 11, 2015 at 14:25 | history | answered | mdperry | CC BY-SA 3.0 |