Timeline for Low temperature PCR
Current License: CC BY-SA 3.0
11 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| Sep 21, 2017 at 8:52 | answer | added | Johnny | timeline score: 0 | |
| Jan 16, 2013 at 11:00 | vote | accept | bobthejoe | ||
| Nov 29, 2012 at 23:18 | answer | added | shigeta | timeline score: 1 | |
| Nov 29, 2012 at 19:10 | answer | added | Royco | timeline score: 1 | |
| Nov 28, 2012 at 23:08 | comment | added | user560 | I've seen PCR melt temps as low as 92 C. It's too low a temp to melt, but do they become fluid > 90 C? | |
| Nov 28, 2012 at 18:51 | comment | added | bobthejoe | If there was an easy solution, I wouldn't be asking it on SE. | |
| Nov 28, 2012 at 15:39 | comment | added | user560 | That's true, but there is a diminishing return on additives (at least DMSO) in which after a certain threshold either the polymerase will no longer function or the reaction will shift towards non-specific priming. | |
| Nov 28, 2012 at 7:49 | history | tweeted | twitter.com/#!/StackBiology/status/273695003753193472 | ||
| Nov 28, 2012 at 6:18 | comment | added | bobthejoe | Well you can change the melting temperature using additives like DMSO and betaine. Unfortunately, the beads have to be coated and the polystyrene seems to be the best for the downstream steps so we would prefer to stick with polystyrene before re-optimizing everything else. | |
| Nov 28, 2012 at 2:58 | comment | added | user560 | Unfortunately melting temperature of nucleic acid is an intrinsic property of the molecule. Do you need to use polystyrene as a substrate? Perhaps silicon or glass are better suited for this in situ PCR. | |
| Nov 27, 2012 at 21:06 | history | asked | bobthejoe | CC BY-SA 3.0 |