Timeline for How can the melting temperature of PCR primers be so far below the extension temperature?
Current License: CC BY-SA 3.0
3 events
| when toggle format | what | by | license | comment | |
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| Jul 5, 2017 at 15:22 | answer | added | rbrucep | timeline score: 2 | |
| Jul 3, 2017 at 7:41 | comment | added | Joe Healey | That will happen in some cases, but by the time you are ready to extend, the annealing step will have mean that primers formed double helix stretches with you template, and the polymerase will have bound to both, stabilising it, thus it isn't free to be released from the strand. | |
| Jul 3, 2017 at 4:53 | history | asked | Arcadium | CC BY-SA 3.0 |