If we were to use a Flow cytometry can it seperate either the xx or xy chromosomes from a sperm

A sperm is haploid. It contains either Y or X but not XY or XX.

Sperm sorting is the process of sorting sperm cells based on specific trait of interest. Whether the sperm is carrying a X or a Y chromosome is definitely of interest to many.

There are a number of methods for sperm sorting (see wiki > sperm sorting > methods), one of which is indeed flow cytometry. Flow cytometry is typically used to seperate X bearing sperms form Y bearing sperms. You should typically have a look at Beltsfield Sperm Sexing Technology (Johson et al. 1999).

If we were to use a flow cytometry, can it separate either the father or mother chromosomes from a sperm?

A sperm is haploid. It contains either Y or X but not XY or XX.

Sperm sorting is the process of sorting sperm cells based on specific trait of interest. Whether the sperm is carrying a X or a Y chromosome is definitely of interest to many.

There are a number of methods for sperm sorting (see wiki > sperm sorting > methods), one of which is indeed flow cytometry. Flow cytometry is typically used to separate X bearing sperms form Y bearing sperms. You should typically have a look at Beltsfield Sperm Sexing Technology (Johson et al. 1999).

If we were to use a Flow cytometry can it seperate either the xx or xy chromosomes from a sperm

A sperm is haploid. It contains either Y or X but not XY or XX.

Sperm sorting is the process of sorting sperm cells based on specific trait of interest. Whether the sperm is carrying a X or a Y chromosome is definitely of interest to many.

There are a number of methods for sperm sorting (see wiki > sperm sorting > methods), one of which is indeed flow cytometry. Flow cytometry is typically used to seperate X bearing sperms form Y bearing sperms. You should typically have a look at Beltsfield Sperm Sexing Technology (Johson et al. 1999).

If we were to use a Flow cytometry can it seperate either the xx or xy chromosomes from a sperm

A sperm is haploid. It contains either Y or X but not XY or XX.

Sperm sorting is the process of sorting sperm cells based on specific trait of interest. Whether the sperm is carrying a X or a Y chromosome is definitely of interest to many.

There are a number of methods for sperm sorting (see wiki > sperm sorting > methods), one of which is indeed flow cytometry. Flow cytometry is typically used to seperate X bearing sperms form Y bearing sperms. You should typically have a look at Beltsfield Sperm Sexing Technology (Johson et al. 1999).