Timeline for What makes/breaks the hydrogen bonds between DNA and RNA during transcription?
Current License: CC BY-SA 4.0
15 events
| when toggle format | what | by | license | comment | |
|---|---|---|---|---|---|
| S Nov 14, 2018 at 2:08 | history | bounty ended | Mesentery | ||
| S Nov 14, 2018 at 2:08 | history | notice removed | Mesentery | ||
| Nov 12, 2018 at 18:00 | history | tweeted | twitter.com/StackBiology/status/1062042441082114051 | ||
| S Nov 12, 2018 at 15:39 | history | bounty started | Mesentery | ||
| S Nov 12, 2018 at 15:39 | history | notice added | Mesentery | Reward existing answer | |
| Nov 12, 2018 at 14:18 | comment | added | David | Finally done it. Perhaps a bit over the top for a non-chemical biologist (as I assume from your bio) or for teaching at certain levels (as you mentioned previously). However as you have made frequent contributions to SE Biology your question deserves a thorough answer. | |
| Nov 12, 2018 at 14:15 | answer | added | David | timeline score: 3 | |
| Nov 8, 2018 at 3:06 | history | edited | theforestecologist♦ | CC BY-SA 4.0 |
deleted 1 character in body
|
| Nov 3, 2018 at 15:11 | history | edited | theforestecologist♦ | CC BY-SA 4.0 |
added 1 character in body
|
| Nov 2, 2018 at 18:56 | answer | added | Stephen B. | timeline score: 5 | |
| Nov 2, 2018 at 18:18 | comment | added | David | I am away with just my phone at the moment which is not really the thing for literature searches etc. If nobody else has done so by next week I'll either do a Papers search for reviews and/or look at recent PDB entries for RNApol. However remember that hydrogen bond formation is driven by thermodynamic considerations at a rate that is governed by kinetic factors. If RNApol makes additional interactions with the rNTP and binds it so it is in the right position and configuration to base pair with the DNA this will allow it to speed up the kinetics — catalyse the reaction. | |
| Nov 2, 2018 at 13:17 | history | edited | theforestecologist♦ | CC BY-SA 4.0 |
added 8 characters in body
|
| Nov 2, 2018 at 13:16 | comment | added | theforestecologist♦ | David, thanks for the comment. I think you're right that all those components are an important part of the process, but I am most interested in the construction/breakdown of the H bonds themselves. I'd welcome an answer that addresses the H-bonds in the context you indicate. Do you have a citation or title for the review paper you have in mind? I'll allow your comment to provide the caution regarding the unwound nature of my animation, but I think I'll leave it as part of my "evidence" that H-bonding occurs and as a quick indication of the H-bond to which I'm referring. Thanks! | |
| Nov 2, 2018 at 12:39 | comment | added | David | Be careful with animations. The one you present indicates an unwound stretch of DNA with RNApol (not shown) somehow moving along it. Misleading at the very least if RNApol is causing the DNA to unwind. I'd remove it. The formation of a transient heteroduplex of at least one bp is fundamental. It seems to me that the crux of your question should be 1. How does RNApol melt the dsDNA, 2. How does it process along the DNA duplex, and 3. What causes the transient heteroduplex of the growing RNA chain to dissociate? The answer is presumably to be found in a recent review paper. | |
| Nov 2, 2018 at 3:33 | history | asked | theforestecologist♦ | CC BY-SA 4.0 |