Timeline for Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?
Current License: CC BY-SA 3.0
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May 31, 2013 at 16:27 | comment | added | MattDMo | @AlanBoyd thanks for the edits - sorry about mitosis, like I said I'm a mammalian guy :) | |
May 31, 2013 at 16:25 | comment | added | Alan Boyd | Also bear in mind that being based on the F plasmid, BACs are low copy-number plasmids. This means you may have to take care with antibiotic concentrations, and if levels of gene expression are important in your work you shouldn't expect as much as you would get from a standard high copy number plasmid. | |
May 31, 2013 at 16:22 | history | edited | Alan Boyd | CC BY-SA 3.0 |
a couple of pedantic edits, plus I don't think it is correct to refer to mitosis in bacteria
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May 31, 2013 at 13:53 | history | answered | MattDMo | CC BY-SA 3.0 |