Timeline for Does precipitation influence the UV spec reading?
Current License: CC BY-SA 3.0
13 events
| when toggle format | what | by | license | comment | |
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| Jun 4, 2013 at 4:46 | comment | added | venkata krishna | Yes UV promotes and it can be read in 230 nm as I have referred earlier. | |
| Jun 4, 2013 at 3:41 | comment | added | venkata krishna | No.. precipitate is forming when sample is added.. | |
| Jun 3, 2013 at 16:29 | comment | added | Alan Boyd | Perhaps you could help us by explaining the basis of the assay? Am I correct in thinking that UV promotes OH radical formation? And that hydrogen-bonded OH radical absorbs at 230 nm? So is the UV beam from the spectrophotometer both promoting radical formation and also allowing the measurement of how much there is of it? And finally, do you get the precipitate if you leave out the peroxide? | |
| Jun 3, 2013 at 15:52 | comment | added | Mad Scientist | @MattDMo UV spectroscopy is a very common technique in the biolab, so this is certainly on-topic here. Migration is rather disruptive, so we try to avoid it for questions that are not actually off-topic on the source site. | |
| Jun 3, 2013 at 15:14 | history | edited | venkata krishna | CC BY-SA 3.0 |
added 368 characters in body
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| Jun 3, 2013 at 14:50 | comment | added | MattDMo | this question would be much more suited to Chemistry.SE | |
| Jun 3, 2013 at 11:42 | answer | added | Alan Boyd | timeline score: 2 | |
| Jun 3, 2013 at 10:24 | history | edited | Rory M | CC BY-SA 3.0 |
Removed noise and corrected a bit of grammar
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| Jun 3, 2013 at 9:38 | comment | added | WYSIWYG | also.. please describe your protocol so that the problem can be understood better | |
| Jun 3, 2013 at 9:04 | comment | added | WYSIWYG | what is your sample and how is it stored ? Is it DNA dissolved in TE/water or cell extracts ? | |
| Jun 3, 2013 at 8:21 | review | Close votes | |||
| Jun 4, 2013 at 12:30 | |||||
| Jun 3, 2013 at 7:30 | review | First posts | |||
| Jun 3, 2013 at 10:24 | |||||
| Jun 3, 2013 at 7:15 | history | asked | venkata krishna | CC BY-SA 3.0 |