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Sep 23, 2019 at 12:14 comment added David And the Sanger method didn't use capilliary gel electrophoresis for many years after its inception. The principle is merely separation, which in a single small laboratory is proably still done by slab gel electrophoresis.
Sep 13, 2019 at 0:29 vote accept user9807
Sep 12, 2019 at 22:05 comment added canadianer Additionally, the original method of Sanger didn’t use labelled ddNTPs at all. The sequence was determined solely on the basis of fragment size and knowledge of which ddNTP terminated it.
Sep 12, 2019 at 13:18 history answered Chris CC BY-SA 4.0